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Identification of Post-translational Modifications of Plant Protein Complexes

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

Bases estructurales para el montaje y la función de un receptor inmune vegetal heterodímero

Simon J Williams¹, Kee Hoon Sohn, Li Wan

¹School of Chemistry and Molecular Biosciences and Australian Infectious Diseases Research Centre, University of Queensland, Brisbane, QLD 4072, Australia.

Science (New York, N.Y.)

|April 19, 2014

Resumen

Los receptores inmunes vegetales RPS4 y RRS1 se asocian físicamente para formar un complejo funcional. Este complejo de receptores es esencial para la defensa de las plantas contra los patógenos, con funciones distintas en el reconocimiento y la señalización.

Área de la Ciencia:

Inmunidad vegetal La inmunidad vegetal.
Mecanismos moleculares de la defensa de las plantas.
Biología estructural de los receptores inmunes.

Sus antecedentes:

Los receptores inmunes de las plantas citoplasmáticas detectan efectores de patógenos para desencadenar la defensa.
RPS4 y RRS1 son receptores inmunes esenciales de Arabidopsis para la defensa contra múltiples patógenos.

Objetivo del estudio:

Investigar la asociación física y la interacción funcional de RPS4 y RRS1.
Para aclarar las bases estructurales de la formación y función del complejo RPS4-RRS1.

Principales métodos:

Coinmunoprecipitación para mostrar la asociación física de RPS4 y RRS1.
Cristalografía de rayos X para determinar las estructuras de los dominios RPS4 y RRS1 TIR.
Análisis de la interfaz de heterodimerización de dominio TIR y su papel en la señalización de defensa.

Principales resultados:

RPS4 y RRS1 se asocian físicamente y forman un complejo heterodímero.
Las estructuras cristalinas revelan una interfaz de interacción TIR/TIR conservada crítica para la formación de complejos.
La heterodimerización del dominio TIR es necesaria para un complejo de reconocimiento de efectores funcionales.
El dominio RPS4 TIR media la defensa independiente del efector, mientras que el dominio RRS1 TIR lo inhibe a través de la interfaz de heterodimerización.

Conclusiones:

RPS4 y RRS1 funcionan como un complejo receptor crucial en la inmunidad de las plantas.
Los roles distintos de RPS4 y RRS1 dentro del complejo están definidos por su interfaz de interacción.
Las ideas estructurales sobre la heterodimerización del dominio TIR proporcionan una base para comprender la función de los receptores inmunes de las plantas.

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