Video Experimental Relacionado
Updated: Jul 12, 2026
11:44
A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
Published on: January 24, 2016
Factores estructuralmente similares pero funcionalmente distintos, IRF-1 e IRF-2, se unen a los mismos elementos
H Harada1, T Fujita, M Miyamoto
1Institute for Molecular and Cellular Biology, Osaka University, Japan.
Cell
|August 25, 1989
Resumen
Los investigadores descubrieron IRF-2, una nueva proteína que interactúa con las mismas secuencias de ADN que IRF-1. El IRF-2 actúa como un supresor, oponiéndose al IRF-1.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- Las infecciones virales desencadenan la expresión génica del interferón tipo I (IFN).
- Este proceso implica la activación de la transcripción por factores como la unión de IRF-1 a los elementos reguladores del ADN.
- La inducción del gen IFN es típicamente transitoria.
Objetivo del estudio:
- Identificar y caracterizar nuevos factores involucrados en la regulación del gen de la interferona.
- Comprender la interacción entre los factores recién descubiertos y los elementos regulatorios conocidos.
- Para dilucidar las funciones opuestas de estos factores en la transcripción génica.
Principales métodos:
- Aislamiento de un ADNc que codifica un nuevo factor, designado IRF-2.
- Análisis de la interacción de IRF-2 con las secuencias de ADN reconocidas por IRF-1.
- Investigar los efectos funcionales de la IRF-2 en la activación génica, particularmente en relación con la IRF-1.
Principales resultados:
- Se aisló un nuevo factor, IRF-2, y se encontró que se une a los mismos elementos cis-activos que IRF-1.
- Tanto la expresión génica de IRF-1 como la de IRF-2 son inducibles por virus y por IFN.
- IRF-2 funciona como un supresor, inhibiendo la función de activación de IRF-1 bajo condiciones específicas.
Conclusiones:
- La transcripción del gen inducible por interferón e IFN está regulada por dos factores de acción trans similares, IRF-1 e IRF-2.
- Estos factores compiten por las mismas secuencias de reconocimiento de ADN.
- IRF-1 e IRF-2 poseen efectos opuestos, lo que sugiere un mecanismo de regulación finamente afinado para las respuestas inmunes.
Videos de Conceptos Relacionados
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
02:26NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
01:31Regulation of the Unfolded Protein Response
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...