Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

6.7K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.7K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

8.4K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
8.4K
Nuclear Export01:42

Nuclear Export

5.3K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.3K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

5.2K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
5.2K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.5K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

18.4K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
18.4K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Indistinguishable mitochondrial phenotypes after exposure of healthy myoblasts to myalgic encephalomyelitis/chronic fatigue syndrome or control serum.

PloS one·2026
Same author

baal-nf identifies motif-disrupting variants that decrease transcription factor binding affinity.

Genome biology·2026
Same author

Epistatic contributions to human traits via transcription factor mechanisms.

medRxiv : the preprint server for health sciences·2025
Same author

Semiparametric efficient estimation of small genetic effects in large-scale population cohorts.

Biostatistics (Oxford, England)·2025
Same author

Replicated blood-based biomarkers for myalgic encephalomyelitis not explicable by inactivity.

EMBO molecular medicine·2025
Same author

Unequal access to diagnosis of myalgic encephalomyelitis in England.

BMC public health·2025

Video Experimental Relacionado

Updated: Apr 10, 2026

Heterokaryon Technique for Analysis of Cell Type-specific Localization
09:31

Heterokaryon Technique for Analysis of Cell Type-specific Localization

Published on: March 11, 2011

17.1K

Un código para RanGDP vinculante en repeticiones de ankyrin define una ruta de importación nuclear.

Min Lu1, Jaroslav Zak1, Shuo Chen1

  • 1Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, OX3 7DQ, UK.

Cell
|May 27, 2014
PubMed
Resumen

Los científicos descubrieron una nueva vía de importación nuclear para las proteínas de repetición de ankyrin (ARPs). Un código específico que involucra residuos hidrofóbicos dirige las ARP al núcleo independientemente de las importinas, impactando el melanoma y la regulación transcripcional.

Más Videos Relacionados

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

9.6K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.9K

Videos de Experimentos Relacionados

Last Updated: Apr 10, 2026

Heterokaryon Technique for Analysis of Cell Type-specific Localization
09:31

Heterokaryon Technique for Analysis of Cell Type-specific Localization

Published on: March 11, 2011

17.1K
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

9.6K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

5.9K

Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Biología celular Biología celular.
  • Genética La genética.

Sus antecedentes:

  • La importación nuclear es crucial para la función de las células eucariotas.
  • La mayoría de las proteínas nucleares utilizan vías mediadas por importinas.
  • Se necesita una vía independiente de la importina para las proteínas que carecen de interacción con la importina.

Objetivo del estudio:

  • Identificar una vía general de importación nuclear independiente de las importaciones.
  • Para definir el código que rige la importación nuclear de las proteínas de repetición de la anquirina (ARPs).
  • Investigar el papel de esta vía en la enfermedad y la regulación transcripcional.

Principales métodos:

  • Identificación de un código específico de aminoácidos dentro de las repeticiones de ankyrin (AR).
  • Pruebas experimentales del código en 17 diferentes ARP.
  • Predicción de la localización nuclear-citoplasmática para más de 150 ARPs humanos anotados.

Principales resultados:

  • Un residuo hidrofóbico en la posición 13 de ARs consecutivos media la unión eficiente a RanGDP.
  • Esta interacción facilita la entrada en el mercado nuclear independiente de las importaciones.
  • El código identificado predice con precisión la localización de ARP y está vinculado a las mutaciones CDKN2A en el melanoma.

Conclusiones:

  • La vía RanGDP/AR (RaDAR) es un mecanismo general de importación nuclear independiente de las importaciones.
  • Esta vía es utilizada con frecuencia por los ARP, en particular los reguladores transcripcionales de NF-κB/p53.
  • El descubrimiento tiene implicaciones para la comprensión de la patogénesis del melanoma y la regulación génica.