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Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
Published on: January 20, 2014
El gen de doble sexo de Drosophila controla la diferenciación sexual somática mediante la producción de ARNm
1Department of Biological Sciences, Stanford University, California 94305.
Cell
|March 24, 1989
Resumen
El gen de doble sexo (dsx) en las moscas de la fruta utiliza un empalme alternativo para crear ARN mensajeros específicos masculinos y femeninos. Este proceso es crucial para regular la diferenciación sexual en ambos sexos.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El gen del doble sexo (dsx) es un regulador clave de la diferenciación sexual somática en Drosophila melanogaster.
- Dos productos funcionales distintos del gen dsx son responsables de reprimir la diferenciación masculina o femenina dependiendo del sexo.
Objetivo del estudio:
- Aclarar los mecanismos moleculares subyacentes a la regulación específica por sexo del gen de doble sexo (dsx).
- Investigar cómo el empalme alternativo y la poliadenilación contribuyen a la producción de ARNm dsx específicos masculinos y femeninos.
Principales métodos:
- Análisis del procesamiento primario de transcripción, incluido el empalme alternativo y la poliadenilación.
- Caracterización genética y molecular de los elementos reguladores involucrados en la expresión dsx.
- Comparación de las estructuras específicas de ARNm masculino y femenino y los polipéptidos codificados.
Principales resultados:
- El gen dsx produce una transcripción primaria común que se empalma alternativamente en ARNm específicos del sexo.
- Estos ARNm comparten exones 5' comunes, pero utilizan exones 3' alternativos, lo que da como resultado terminales carboxilo específicos del sexo de las proteínas codificadas.
- Los datos genéticos implican secuencias cerca del sitio aceptor de empalme específico de la hembra en la regulación de la expresión dsx a través de los loci transformador y transformador-2.
Conclusiones:
- El empalme alternativo de la transcripción primaria dsx es el mecanismo que genera proteínas funcionales específicas del sexo.
- Los exones 3 'específicos del sexo dictan los roles distintos de dsx en la diferenciación sexual masculina y femenina.
- Los genes transformador y transformador-2 juegan un papel regulador en el empalme específico por sexo del ARNm dsx.
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