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Los poros de polímero modificados por ADN permiten el control de pH y voltaje del flujo de canal
Steven F Buchsbaum1, Gael Nguyen, Stefan Howorka
1School of Physical Sciences, University of California , Irvine, California 92697, United States.
Journal of the American Chemical Society
|July 4, 2014
Resumen
Los investigadores crearon nanoporos sintéticos inteligentes que imitan los canales biológicos. Estos poros basados en ADN responden a los cambios de pH y voltaje, lo que permite aplicaciones avanzadas de biosensores y administración de fármacos.
Área de la Ciencia:
- Ciencia de los materiales Ciencia de los materiales.
- Biotecnología La biotecnología es la biotecnología.
- Nanotecnología La nanotecnología es la nanotecnología.
Sus antecedentes:
- Los canales iónicos biológicos controlan el transporte celular a través de estímulos como el pH y el voltaje.
- La imitación de estos canales es clave para las membranas inteligentes en el biosensing y la administración de medicamentos.
Objetivo del estudio:
- Desarrollar nanoporos sintéticos con doble capacidad de respuesta al pH y potencial transmembrana.
- Para diseñar membranas inteligentes para aplicaciones avanzadas.
Principales métodos:
- La fijación de oligómeros de ADN con bases protonables a las aberturas asimétricas de los nanoporos.
- Utilizando la formación de malla electrostática inducida por el pH para el cierre de poros.
- Empleando la conmutación de voltaje para el movimiento nanomecánico y la modulación de corriente.
Principales resultados:
- Se lograron resistencias de poro sin precedentes (decenas de gigaohms) a pH 5.5 debido a la formación de malla electrostática del ADN.
- Modulación de corriente reversible demostrada a través del movimiento nanomecánico del ADN a pH neutro y con cambios de voltaje.
- Confirmó la robustez del mecanismo de cierre reversible dependiente del pH para nanoporos de hasta 14 nm.
Conclusiones:
- Se demostró con éxito un concepto novedoso para el encierro de nanoporos sintéticos basado en estímulos de pH y voltaje.
- El mecanismo de malla electrostática ofrece un método robusto para controlar el transporte de iones en los nanoporos.
- Este enfoque tiene aplicaciones potenciales en biosensing, administración de fármacos y circuitos iónicos, adaptables a varios polímeros orgánicos.
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