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Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

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Una red de interacción de acompañante cuantitativa revela la arquitectura de las vías de homeostasis de las proteínas

Mikko Taipale¹, George Tucker², Jian Peng²

¹Whitehead Institute for Biomedical Research, Cambridge, MA 02114, USA.

|July 19, 2014

Resumen

Las chaperonas y cochaperonas celulares regulan el plegamiento de las proteínas. Este estudio traza un mapa de sus extensas redes de interacción, revelando las funciones de la cochaperona en la homeostasis de las proteínas y su potencial como sensores de drogas objetivo.

Área de la Ciencia:

Biología celular Biología celular.
La bioquímica de las proteínas.

Sus antecedentes:

Las chaperonas ayudan al plegamiento y la función de las proteínas.
Las cocaperonas modulan la actividad de las chaperonas, pero sus funciones son poco conocidas.
La complejidad de la red chaperón-cochaperón-cliente y las funciones independientes del chaperón-cochaperón siguen siendo en gran medida desconocidas.

Objetivo del estudio:

Caracterizar sistemáticamente la red de interacción chaperón-cochaperón-cliente en las células humanas.
Para delinear la participación de cocaperón en complejos específicos.
Para explorar las funciones biológicas independientes de la cochaperona.

Principales métodos:

Ensayos LUMIER cuantitativos de alto rendimiento.
Espectrometría de masas. espectrometría de masas.
Caracterización sistemática de las interacciones proteína-proteína.

Principales resultados:

Se identificaron cientos de clientes acompañantes.
Se establecieron distintas redes de interacción proteína-proteína para las cochaperonas.
Se demostró que las cochaperonas de la familia NUDC se asocian con pliegues específicos de la hélice β.

Conclusiones:

Se proporcionó un marco para descifrar la red de proteostasis y su regulación en el desarrollo y la enfermedad.
Los chaperones se pueden utilizar como sensores para el compromiso con el objetivo de la droga.
Los cocaperones poseen distintas redes de interacción y funciones potencialmente independientes de la chaperona.

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