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Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
Published on: July 30, 2010
Análisis bioquímico de la activación transcripcional por Jun: actividad diferencial de c- y v-Jun
Cell
|November 17, 1989
Resumen
El estudio compara los factores de transcripción humanos c-Jun y virales v-Jun. V-Jun muestra una mayor actividad debido a un dominio regulador eliminado en c-Jun, lo que afecta a la regulación de la expresión génica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La oncogénesis es la oncogénesis.
- Reglamento genético Reglamento genético.
Sus antecedentes:
- c-Jun es un producto proto-oncogénico humano y miembro de la familia de factores de transcripción AP-1.
- Los factores de transcripción AP-1 regulan la expresión génica en respuesta a las señales extracelulares.
Objetivo del estudio:
- Para comparar la actividad transcripcional del virus humano c-Jun y el viral v-Jun.
- Identificar los dominios reguladores responsables de las diferencias en la actividad transcripcional.
Principales métodos:
- Se utilizaron ensayos de transcripción in vitro para comparar c-Jun y v-Jun.
- Se realizó un análisis de mutantes Jun expresados en bacterias.
Principales resultados:
- v-Jun exhibió una actividad transcripcional significativamente mayor que c-Jun.
- Se identificó un dominio regulador en la región N-terminal de c-Jun como responsable de la diferencia de actividad.
- Se encontró un dominio de activación rico en residuos ácidos y de prolina cerca del dominio de unión del ADN C-terminal.
Conclusiones:
- La transducción retroviral probablemente generó una proteína Jun constitutivamente activa al eliminar un dominio de acción negativa.
- Este supuesto dominio represor también puede estar involucrado en la inducción dependiente de la señal de la actividad c-Jun.
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