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Activador de transcripción putativo con isoformas alternativas codificadas por el gen ZFX humano
A Schneider-Gädicke1, P Beer-Romero, L G Brown
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Nature
|December 7, 1989
Resumen
El gen ZFX, similar al gen ZFY ligado a Y, puede desempeñar un papel en la determinación del sexo humano. Codifica proteínas activadoras de transcripción con dominios funcionales distintos, lo que sugiere una variedad de roles en el desarrollo.
Área de la Ciencia:
- Genética La genética.
- Biología Molecular Biología Molecular
- Biología del desarrollo Biología del desarrollo.
Sus antecedentes:
- El gen ZFY en el cromosoma Y está implicado en la determinación del sexo humano.
- El gen ZFX en el cromosoma X comparte similitudes con ZFY y escapa a la inactivación de X.
- Tanto ZFY como ZFX probablemente evolucionaron de un gen ancestral común.
Objetivo del estudio:
- Para caracterizar el producto proteico del gen ZFX.
- Para investigar el papel potencial de ZFX en la determinación del sexo.
- Para explorar las implicaciones funcionales de las isoformas de proteínas ZFX.
Principales métodos:
- Análisis de ADNs complementarios (ADNc). análisis de ADNs complementarios (ADNc). análisis de ADNs complementarios (ADNc). análisis de ADNs complementarios (ADNc).
- Análisis del dominio de la proteína.
- Investigación del empalme alternativo.
Principales resultados:
- ZFX codifica una proteína con un dominio amino-terminal ácido, una señal de localización nuclear y un dominio de dedo de zinc.
- Estas características son características de los activadores de transcripción, similares a las de ZFY.
- El empalme alternativo produce isoformas ZFX de 575 y 804 aminoácidos, que difieren en la longitud del dominio ácido.
Conclusiones:
- La proteína ZFX posee características típicas de los activadores de la transcripción.
- El gen ZFX puede funcionar en la determinación del sexo, de manera análoga a ZFY.
- El empalme alternativo genera isoformas de proteínas ZFX funcionalmente distintas.
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