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Los Riboswitches. también conocidos como Riboswitches. Un sRNA que contiene riboswitch controla la expresión génica

Sruti DebRoy¹, Margo Gebbie², Arati Ramesh³

¹Department of Microbiology and Molecular Genetics, The University of Texas Health Science Center at Houston, TX 77030, USA.

Science (New York, N.Y.)

|August 23, 2014

Resumen

Un nuevo mecanismo de riboswitch controla los genes de utilización de la etanolamina en el Enterococcus faecalis. El ARN EutX secuestra el regulador EutV en ausencia de adenosil cobalamina (AdoCbl), lo que afecta la expresión génica.

Área de la Ciencia:

Microbiología Microbiología.
Biología Molecular Biología Molecular
La bioquímica es la bioquímica.

Sus antecedentes:

El locus de utilización de la etanolamina (eut) en Enterococcus faecalis comprende al menos 19 genes.
La expresión génica del locus eut está regulada principalmente por un único riboswitch sensible a la adenosil cobalamina (AdoCbl).

Objetivo del estudio:

Para aclarar el mecanismo de la regulación dependiente de AdoCbl del eut locus.
Caracterizar el papel del ARN EutX y del regulador EutV en el control de los genes posttranscripcionales.

Principales métodos:

Investigó la interacción entre el ARN EutX y el regulador EutV.
Estudió el efecto de AdoCbl en la unión EutX-EutV y la posterior expresión génica.
Analizó el papel de EutX en el secuestro de EutV.

Principales resultados:

El riboswitch de unión a AdoCbl es parte de un ARN de acción trans, EutX.
EutX secuestra el regulador de EutV en ausencia de AdoCbl a través de una estructura de doble horquilla.
En presencia de AdoCbl, se libera EutV y promueve la lectura transcripcional de los genes eut.

Conclusiones:

Se introdujo el control mediado por riboswitch del secuestro de proteínas como un nuevo mecanismo de regulación de genes posttranscripcional.
Se demostró cómo EutX y EutV regulan coordinadamente la expresión de múltiples genes eut en respuesta a AdoCbl.

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