Video Experimental Relacionado
Updated: Apr 25, 2026
10:22
Comprehensive Spatial Profiling of Species-agnostic Transcriptomes via Stereo-seq
Published on: October 31, 2025
908
Análisis comparativo del transcriptoma entre especies distantes
Mark B Gerstein1, Joel Rozowsky2, Koon-Kiu Yan2
11] Program in Computational Biology and Bioinformatics, Yale University, Bass 432, 266 Whitney Avenue, New Haven, Connecticut 06520, USA [2] Department of Molecular Biophysics and Biochemistry, Yale University, Bass 432, 266 Whitney Avenue, New Haven, Connecticut 06520, USA [3] Department of Computer Science, Yale University, 51 Prospect Street, New Haven, Connecticut 06511, USA [4] [5].
Nature
|August 29, 2014
Resumen
La comparación de transcriptomas entre especies revela principios biológicos fundamentales. Este estudio identificó patrones compartidos de expresión génica y un modelo universal para predecir la actividad génica a partir de datos de cromatina en humanos, gusanos y moscas.
Área de la Ciencia:
- La genómica comparada es una genómica comparada.
- La transcriptómica es el campo de la transcripción.
- Biología del desarrollo Biología del desarrollo.
Sus antecedentes:
- El transcriptoma proporciona una instantánea de la actividad del genoma.
- Las comparaciones de transcriptomas entre especies pueden descubrir principios biológicos fundamentales.
- Las comparaciones anteriores se limitaron a especies o filos.
Objetivo del estudio:
- Para identificar las características conservadas en los transcriptomas a través de especies animales distantes (humano, gusano, mosca).
- Desarrollar un modelo universal para predecir la expresión génica a partir de datos de cromatina.
- Para comparar los niveles de transcripción no codificantes entre especies.
Principales métodos:
- Generación de datos de secuenciación de ARN y procesamiento uniforme por parte de los consorcios ENCODE y modENCODE.
- Análisis comparativo de transcriptomas en humanos, gusanos y moscas.
- Desarrollo y aplicación de un modelo predictivo utilizando las características de la cromatina.
Principales resultados:
- Descubrimiento de módulos de coexpresión compartidos, muchos enriquecidos en genes de desarrollo, en todo el filo metazoario.
- Identificación de un nuevo alineamiento en la etapa de desarrollo entre pupas de gusanos y embriones de moscas.
- Cuantificación de niveles de transcripción no canónicos similares por par de bases entre especies.
- Demostración de que los niveles de expresión génica pueden predecirse a partir de las características de la cromatina promotora utilizando un modelo independiente del organismo.
Conclusiones:
- Las comparaciones de transcriptomas entre diversas especies revelan características antiguas y conservadas.
- Los procesos de desarrollo muestran principios reguladores conservados en todos los animales.
- Un modelo universal puede predecir la expresión génica a partir de datos de cromatina en todas las especies, destacando los mecanismos reguladores conservados.
Videos de Conceptos Relacionados
02:54Evolutionary Relationships through Genome Comparisons
5.8K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.8K
02:24Ribosome Profiling
3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K
02:16Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
11.5K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
11.5K
02:05Gene Evolution - Fast or Slow?
6.2K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
6.2K
02:51Multi-species Conserved Sequences
3.3K
Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
3.3K
02:26Comparing Copy Number Variations and SNPs
11.4K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
11.4K