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La estructura compleja del péptido de cobre y la reactividad cuando se encuentra en las secuencias His-X(aa) -His

Ga Young Park¹, Jung Yoon Lee, Richard A Himes

¹Department of Chemistry, The Johns Hopkins University , Baltimore, Maryland 21218, United States.

Journal of the American Chemical Society

|August 30, 2014

Resumen

Las preferencias tautoméricas en las proteínas de cobre que contienen histidina alteran significativamente el cobre.

Área de la Ciencia:

Química bioorgánica Química bioorgánica.
Coordinación Química de la Coordinación
Química de las proteínas química de las proteínas.

Sus antecedentes:

Las proteínas de cobre que activan el oxígeno a menudo presentan motivos quelantes His-X(aa) -His.
Los anillos de imidazol en estas proteínas pueden existir en formas tautoméricas (δN vs. εN), influyendo en las interacciones de iones de cobre.

Objetivo del estudio:

Investigar cómo las preferencias tautoméricas δN vs. εN de los residuos de histidina afectan la coordinación de iones de cobre, las propiedades redox y la reactividad.
Para sintetizar y caracterizar el cobre ((I) complejos con δ-HGH y ε-HGH tripeptídeos.

Principales métodos:

La espectroscopia de absorción de rayos X es una espectroscopia de absorción de rayos X.
Cálculos de la teoría funcional de densidad cálculos de la teoría funcional de densidad.
Mediciones de conductividad de la solución Mediciones de conductividad de la solución.
Síntesis y caracterización de los complejos de cobre (I)

Principales resultados:

δ-HGH formó un complejo dimérico Cu (I) [{Cu (I) (δ-HGH) ) ]2+), mientras que ε-HGH formó un complejo monomérico [Cu (I) (ε-HGH) ]+).
El complejo monomérico [Cu(I) ((ε-HGH) ](+) mostró reactividad, formando un adducto de CO similar a la monooxigenasa de cobre PHM.
Este complejo también reaccionó con O2 y H2O2 para formar nuevos compuestos adictos de O2 o complejos Cu(II) -OOH.

Conclusiones:

Las preferencias tautoméricas de la histidina influyen críticamente en la geometría de coordinación del cobre y la reactividad en los sistemas bioinorgánicos.
El complejo ε-HGH-Cu(I) sirve como un modelo funcional para las monooxigenasas de cobre, destacando la importancia de entornos de coordinación específicos.
Este estudio proporciona información sobre los mecanismos de activación del oxígeno dependiente del cobre y las reacciones de oxidación.

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