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Oromucosal as an Alternative Method for Administration of Cannabis Products in Rodents03:43

Oromucosal as an Alternative Method for Administration of Cannabis Products in Rodents

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Oral administration via gavage in rodents can induce distress associated with restraint. We explored oromucosal/buccal administration of CBD-enriched Cannabis extract, where rats were treated for 15 days with the extract at 3 mg/kg/day. Treatments were administered orally with a micropipette, using an affectionate touch technique to handle the...
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Here, the method of inducing pulmonary arterial hypertension associated with pulmonary fibrosis (PF-PH) rat model by injecting bleomycin into the airway is introduced. We also provide a step-by-step approach to validate this animal...
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This work describes a robust and straightforward method to detect and quantify the endocannabinoid 2-arachidonoylglycerol (2-AG) in C. elegans. An analytical deuterated standard us prepared and used for the quantification of 2-AG by isotopic dilution and liquid chromatography-electrospray ionization-tandem mass spectrometry...
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Here we describe a detailed protocol for invasive measurements of hemodynamic parameters including portal pressure, splanchnic blood flow, and systemic hemodynamics in order to characterize the portal hypertensive syndrome in...
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The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport13:17

The Mesenteric Lymph Duct Cannulated Rat Model: Application to the Assessment of Intestinal Lymphatic Drug Transport

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Here we describe a technique to cannulate the mesenteric lymph duct in rats which enables quantification of lipid and drug transport via the lymphatic system following intestinal delivery. The technique can be adapted to assess mesenteric lymph concentrations and/or transport of fluid, immune cells, peptides, proteins and lipophilic...
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Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis08:56

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Disruption of bile flow results in severe inflammatory cholestatic liver injury with a characteristic time-dependent sequence of morphological alterations. Here we present a protocol for the surgical ligation of the common bile duct in mice that allows to induce a strong fibrotic response after 21 to 28...
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Video Experimental Relacionado

Updated: Jan 11, 2026

Invasive Hemodynamic Characterization of the Portal-hypertensive Syndrome in Cirrhotic Rats
09:37

Invasive Hemodynamic Characterization of the Portal-hypertensive Syndrome in Cirrhotic Rats

Published on: August 1, 2018

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Oceanografía: Lo que baja debe subir.

Raffaele Ferrari1

  • 1Department of Earth, Atmospheric and Planetary Sciences, Massachusetts Institute of Oceanography, Cambridge, Massachusetts 02139, USA.

Nature
|September 12, 2014
PubMed
Resumen

No abstract available in PubMed .

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