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Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

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Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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Pinching-off of Coated Vesicles01:32

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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Vesicular Tubular Clusters01:45

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After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
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Fusion of Secretory Vesicles with the Plasma Membrane01:26

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Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
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Delivery Pathways to the Lysosome01:36

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Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
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Recycling Endosomes and Transcytosis00:58

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The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
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Updated: Apr 22, 2026

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
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La clatrina regenera las vesículas sinápticas de los endosomas.

Shigeki Watanabe1, Thorsten Trimbuch2, Marcial Camacho-Pérez2

  • 1Department of Biology and Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84112-0840, USA.

Nature
|October 9, 2014
PubMed
Resumen
Este resumen es generado por máquina.

La endocitosis ultrarrápida recupera rápidamente las vesículas sinápticas. La clatrina es esencial para la regeneración de las vesículas sinápticas de los endosomas, no para la recuperación inicial ultra rápida.

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Área de la Ciencia:

  • La neurociencia es la neurociencia.
  • Biología celular Biología celular.
  • Biología Molecular Biología Molecular

Sus antecedentes:

  • El reciclaje de las vesículas sinápticas es crucial para la comunicación neuronal.
  • El mecanismo y el momento de la endocitosis de las vesículas sinápticas aún se debaten.
  • La endocitosis ultrarrápida recupera rápidamente las grandes vesículas después de la fusión.

Objetivo del estudio:

  • Para aclarar el destino de las grandes vesículas endocíticas formadas durante la endocitosis ultrarrápida.
  • Determinar el papel de la clatrina y la actina en el reciclaje de las vesículas sinápticas.
  • Para resolver las discrepancias en los estudios publicados sobre el papel de la clatrina en la endocitosis.

Principales métodos:

  • Utilizó la interferencia de ARN (RNAi) para interrumpir la función de la clatrina.
  • La polimerización de actina manipulada y la temperatura de estimulación neuronal.
  • Rastreó la transición de las vesículas endocíticas a las vesículas sinápticas con el tiempo.

Principales resultados:

  • Las grandes vesículas endocíticas maduran en endosomas sinápticos dentro de un segundo.
  • Los endosomas se disuelven en vesículas recubiertas y luego en pequeñas vesículas sinápticas en 5-6 segundos.
  • La clatrina es necesaria para generar vesículas sinápticas de los endosomas, pero no para la endocitosis ultrarrápida.
  • La endocitosis ultrarrápida depende de la polimerización de actina y la temperatura fisiológica.
  • La endocitosis mediada por clatrina sirve como una vía de recuperación alternativa cuando la endocitosis ultrarrápida está deteriorada.

Conclusiones:

  • La endocitosis ultrarrápida es una vía independiente de la clatrina para la recuperación inicial de las vesículas.
  • La endocitosis dependiente de clatrina es esencial para la posterior reformación de las vesículas sinápticas de los endosomas.
  • La polimerización de la actina y la temperatura son factores críticos para la endocitosis ultrarrápida.
  • Los hallazgos aclaran las distintas funciones de la clatrina en diferentes vías de endocitosis de las vesículas sinápticas.