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Imaging G-protein Coupled Receptor GPCR-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

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Published on: September 20, 2011

Regulación y función de las subunidades de la proteína G alfa en Dictyostelium

A Kumagai¹, M Pupillo, R Gundersen

¹Department of Biology, University of California, San Diego, La Jolla 92093.

|April 21, 1989

Resumen

Dos subunidades de proteína G alfa en Dictyostelium, G alpha 1 y G alpha 2, tienen funciones distintas en el desarrollo y la señalización. G alfa 2 media las respuestas inducidas por el cAMP como la quimiotaxis, mientras que G alfa 1 afecta el ciclo celular y el desarrollo.

Área de la Ciencia:

La señalización celular de las células.
Biología del desarrollo Biología del desarrollo.
Genética molecular genética molecular.

Sus antecedentes:

Las proteínas G alfa son transductores de señales clave en los eucariotas.
Dictyostelium discoideum sirve como un organismo modelo para estudiar el desarrollo celular y las vías de señalización.

Objetivo del estudio:

Para investigar la regulación del desarrollo y las funciones funcionales de las subunidades de proteínas G alfa 1 y G alfa 2 en Dictyostelium.
Para aclarar las contribuciones específicas de cada subunidad G alfa a la transducción de señales y procesos de desarrollo.

Principales métodos:

Análisis de los patrones de expresión de G alfa 1 y G alfa 2 durante el desarrollo del Dictyostelium.
Estudios funcionales que involucran sobreexpresión de G alfa 1 para observar las consecuencias del desarrollo.
Evaluación del papel de G alfa 2 en la mediación de la transducción de señales mediada por el receptor cAMP.

Principales resultados:

G alfa 1 se expresa desde las etapas vegetativas hasta las agregadas.
G alfa 2 es inducible por la cAMP y se expresa preferentemente durante la agregación.
G alfa 2 es esencial para la transducción de señales mediada por el receptor cAMP, incluida la quimiotaxis y la regulación génica.
G alfa 1 juega un papel en el ciclo celular y el desarrollo, con una sobreexpresión que conduce a células multinucleadas anormales y defectos de desarrollo.

Conclusiones:

G alfa 2 es el principal mediador de las vías de señalización inducidas por cAMP en Dictyostelium.
G alfa 1 tiene funciones críticas tanto en la progresión del ciclo celular como en los procesos de desarrollo.
Estas proteínas G alfa son fundamentales para comprender la transducción de señales y el control del desarrollo en Dictyostelium.

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