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Estructura e inhibición de EV-D68, un virus que causa enfermedades respiratorias en niños
Yue Liu1, Ju Sheng1, Andrei Fokine1
1Department of Biological Sciences, Hockmeyer Hall of Structural Biology, 240 South Martin Jischke Drive, Purdue University, West Lafayette, IN 47907, USA.
Science (New York, N.Y.)
|January 3, 2015
Resumen
El enterovirus D68 (EV-D68), una causa de enfermedades respiratorias infantiles, fue estudiado utilizando estructuras cristalinas. El medicamento antiviral pleconaril inhibe eficazmente el EV-D68, mostrando potencial para el tratamiento de brotes.
Área de la Ciencia:
- Virología Virología.
- Biología Estructural Biología estructural.
- Descubrimiento de Drogas Descubrimiento de Drogas
Sus antecedentes:
- El enterovirus D68 (EV-D68) es un patógeno significativo que causa brotes de enfermedades respiratorias en niños.
- EV-D68 pertenece a la familia Picornaviridae, lo que requiere investigación sobre su estructura y posibles tratamientos.
Objetivo del estudio:
- Para determinar las estructuras cristalinas del enterovirus D68 (EV-D68) y su complejo con el compuesto antiviral pleconaril.
- Para investigar la interacción de unión del pleconaril dentro de la estructura de la cápside EV-D68.
- Para evaluar la eficacia antiviral del pleconaril contra el EV-D68.8.
Principales métodos:
- Se empleó cristalografía de rayos X para obtener estructuras de alta resolución de EV-D68 y su complejo con pleconaril.
- Análisis de la bolsa hidrofóbica de unión al fármaco en la proteína viral 1.
- Pruebas antivirales in vitro para determinar la concentración efectiva semimaxima (EC50) del pleconaril.
Principales resultados:
- La estructura cristalina reveló una bolsa hidrofóbica en la proteína viral 1 ocupada por un ácido graso.
- Pleconaril desplazó con éxito el ácido graso de la bolsa de unión.
- Pleconaril demostró una potente inhibición de EV-D68 con una EC50 de 430 nanomolares.
Conclusiones:
- Los datos estructurales y bioquímicos aclaran el mecanismo de unión del pleconaril al EV-D68.8.
- Pleconaril es un prometedor candidato a fármaco para el manejo de brotes de Enterovirus D68.
- Un mayor desarrollo del pleconaril podría proporcionar una opción terapéutica para las infecciones por EV-D68.
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