Video Experimental Relacionado
Updated: Apr 18, 2026
10:31
Author Spotlight: Universal Molecular Retention with 11-Fold Expansion Microscopy
Published on: October 6, 2023
9.2K
Imágenes ópticas. imágenes ópticas. Microscopía de expansión por expansión
Fei Chen1, Paul W Tillberg2, Edward S Boyden3
1Department of Biological Engineering, Massachussetts Institute of Technology (MIT), Cambridge, MA, USA.
Science (New York, N.Y.)
|January 17, 2015
Resumen
La microscopía de expansión (ExM) magnifica físicamente las muestras mediante la síntesis de una red de polímero hinchable. Esta técnica permite imágenes escalables de superresolución con microscopios convencionales limitados por difracción, logrando una resolución de ~70 nm.
Área de la Ciencia:
- La biofísica es la biofísica.
- Microscopía óptica y microscopía óptica.
- Ciencia de los materiales Ciencia de los materiales.
Sus antecedentes:
- La microscopía óptica se basa en la refracción para el aumento de imagen.
- Los detalles estructurales finos a menudo están limitados por el límite de difracción óptica.
Objetivo del estudio:
- Desarrollar un método para la microscopía de superresolución escalable utilizando instrumentos de difracción limitada.
- Para superar las limitaciones del límite de difracción óptica para imágenes biológicas.
Principales métodos:
- La síntesis de una red de polímeros hinchables dentro de especímenes biológicos.
- Etiquetas de anclaje covalente a la red de polímeros para la expansión isotrópica.
- Utilizando la ampliación física a través de la expansión de la red de polímeros.
Principales resultados:
- Microscopía de expansión (ExM) demostrada con una resolución lateral de ~70 nanómetros.
- Logró imágenes de superresolución en células cultivadas y tejido cerebral.
- Realizó imágenes de superresolución en tres colores del hipocampo del ratón utilizando un microscopio confocal.
Conclusiones:
- ExM permite la microscopía de superresolución escalable con microscopios convencionales.
- La expansión física supera el límite de difracción óptica para una resolución mejorada.
- ExM es una poderosa técnica para obtener imágenes de alta resolución de estructuras biológicas.
Videos de Conceptos Relacionados
01:18Imaging Biological Samples with Optical Microscopy
12.3K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.3K
01:16Confocal Fluorescence Microscopy
22.2K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.2K
01:22Overview of Microscopy Techniques
17.9K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.9K
01:37Super-resolution Fluorescence Microscopy
14.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.9K
01:26Phase Contrast and Differential Interference Contrast Microscopy
15.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
15.4K
01:28Three-Dimensional Microscopy in Microbiology
1.1K
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
1.1K