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Extensa variación del número de copias a nivel de cepa entre las especies del microbioma intestinal humano
Sharon Greenblum1, Rogan Carr1, Elhanan Borenstein2
1Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.
Cell
|February 3, 2015
Resumen
Las bacterias intestinales humanas exhiben variaciones significativas en el número de copias de genes entre las cepas, lo que afecta la función microbiana y la salud del huésped. Este estudio revela conocimientos clínicamente relevantes sobre esta variación intraespecie utilizando datos metagenómicos.
Área de la Ciencia:
- Microbiología Microbiología.
- La genómica es la genómica.
- La bioinformática es la bioinformática.
Sus antecedentes:
- Las cepas bacterianas dentro de una especie pueden diferir en contenido genético y número de copias.
- El análisis actual de la microbiota humana a menudo pasa por alto la variación intraespecífica, lo que limita la comprensión de sus implicaciones funcionales y de salud.
Objetivo del estudio:
- Para llevar a cabo un análisis a gran escala de la variación del número de copias intraespecie en el microbioma intestinal.
- Desarrollar una tubería computacional para detectar la variación del número de copias de genes a partir de datos metagenómicos de escopeta.
- Para explorar la relevancia funcional y clínica de esta variación.
Principales métodos:
- Desarrollo de una tubería computacional para detectar la variación del número de copias intraespecíficas.
- Análisis de los datos metagenómicos de escopeta del microbioma intestinal humano.
- Inferencia de los perfiles de composición intraespecie y la estructura de la población.
Principales resultados:
- Identificación de un número sustancial de genes variables en numerosas especies bacterianas.
- Demostrar las implicaciones funcionales y clínicamente relevantes de la variación intraespecífica.
- Descubrimiento de cambios en la estructura de poblaciones y variantes no caracterizadas dentro de las especies.
Conclusiones:
- La variación del número de copias de genes intraespecies es prevalente y funcionalmente significativa en el microbioma intestinal.
- El análisis metagenómico puede revelar variaciones a nivel de cepa, ofreciendo una visión más profunda que la caracterización a nivel de especie.
- La composición del microbioma y la capacidad funcional están intrínsecamente vinculadas, influenciadas por la diversidad genética a nivel de cepa.
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