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Dímetros de cadena β mediados por disulfuro: hojas β hiperestables que carecen de interacciones terciarias y giros

Brandon L Kier¹, Jordan M Anderson¹, Niels H Andersen¹

¹Chemistry Department, University of Washington, Seattle, Washington 98195, United States.

Journal of the American Chemical Society

|April 4, 2015

Resumen

Los enlaces disulfuro pueden nuclear la estructura antiparalela de la hoja beta en los péptidos. Este nuevo método, utilizando cistinas centrales de hebra, ofrece una forma superior de diseñar hojas beta estables sin giros tradicionales.

Área de la Ciencia:

Diseño de proteínas y péptidos.
Química biofísica y bioquímica.
Biología Estructural Biología estructural.

Sus antecedentes:

Los enlaces disulfuro son cruciales para la estructura y el plegamiento de las proteínas.
Su uso en el diseño de péptidos ha implicado principalmente el refuerzo de estructuras existentes o la creación de nudos.
Los enlaces de disulfuro en posiciones de no enlace H se han utilizado en horquillas beta para controles positivos.

Objetivo del estudio:

Introducir una nueva clase de dímeros peptídicos de hoja beta diseñados.
Investigar el papel de los disulfuros centrales de cadena como nucleadores de la formación de hojas beta.
Establecer principios de diseño para hojas beta "sin giro" atadas a disulfuro.

Principales métodos:

Diseño y síntesis de péptidos cortos que incorporan enlaces disulfuro centrales.
Caracterización de la nucleación y estabilización de la estructura de la hoja beta.
Análisis comparativo de los enlaces disulfuro frente a los giros tradicionales en la promoción de la formación de hojas beta.

Principales resultados:

Un solo enlace disulfuro cerca del centro de la cadena peptídica puede nuclear una estructura de hoja beta antiparalela.
La adición de unidades de beta-capping e interacciones entre hebras lleva a hojas hiperestables.
Las cistinas centrales de hebra son más efectivas en la formación de hojas beta nucleantes que las vueltas de reversión diseñadas.

Conclusiones:

Los enlaces disulfuro pueden servir como nucleadores efectivos para la estructura de la hoja beta.
Esta estrategia permite el diseño de hojas beta estables y "sin giro".
Proporciona un marco y reglas de diseño para futuras aplicaciones de la formación de hojas beta mediadas por disulfuro.

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