Video Experimental Relacionado

Updated: Apr 15, 2026

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

Published on: December 23, 2021

Los repetidos contactos ER-endosoma promueven la translocación endosómica y el crecimiento de neuritas

Camilla Raiborg¹, Eva M Wenzel¹, Nina M Pedersen¹

¹1] Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N-0379 Oslo, Norway [2] Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, N-0379 Oslo, Norway.

|April 10, 2015

Resumen

La protrudina forma el retículo endoplasmático (ER) y los sitios de contacto del endosoma tardío (LE), lo que facilita la transferencia de la quinesina-1. Este proceso impulsa a las LEs a la periferia celular, promoviendo la protuberancia y el crecimiento de las neuritas.

Área de la Ciencia:

Biología celular Biología celular.
Biología Molecular Biología Molecular
La neurociencia es la neurociencia.

Sus antecedentes:

El retículo endoplasmático (ER) y los endosomas se conectan a través de sitios de contacto, cruciales para los procesos celulares.
Los mecanismos y funciones precisos de los sitios de contacto del endosoma ER no se comprenden completamente.
Se sabe que la protrudina, una proteína ER, influye en las protuberancias celulares y el crecimiento de neuritas.

Objetivo del estudio:

Para aclarar el papel de la protrudina en los sitios de contacto del endosoma ER.
Investigar cómo los contactos ER-endosoma regulan el tráfico endosómico y la morfología celular.
Para identificar los actores moleculares involucrados en las interacciones ER-endosoma mediadas por protrudina.

Principales métodos:

Utilizó líneas celulares humanas y de ratas.
Interacciones de proteínas investigadas utilizando ensayos de coinmunoprecipitación y ligadura por proximidad.
Utilizó imágenes de células vivas para rastrear el movimiento del endosoma y los contactos ER-endosoma.
Utilizó siRNA y CRISPR/Cas9 para agotar o eliminar las proteínas clave.

Principales resultados:

La protrudina forma sitios de contacto con los endosomas tardíos (LEs) mediante el reconocimiento de RAB7 y fosfatidilinositol 3-fosfato (PtdIns(3) P).
Estos sitios de contacto facilitan la transferencia de la proteína motora kinesin-1 de la protrudina a la FYCO1 en las LEs.
Los contactos repetidos de ER-LE promueven la translocación de LE impulsada por la quinesina-1 a la periferia celular, lo que lleva a la fusión de la membrana plasmática, la protuberancia y el crecimiento de neuritas.

Conclusiones:

Los sitios de contacto ER-LE mediados por protrudina actúan como plataformas para la carga de kinesin-1 en las LEs.
El transporte de LE dependiente de la kinesin-1, impulsado por los contactos de ER, es esencial para la protuberancia y el crecimiento de neuritas.
Este estudio revela un nuevo mecanismo que vincula la dinámica de contacto ER-endosoma con el desarrollo neuronal y la morfología celular.

Más Videos Relacionados

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

Quantitative Approaches for Scoring in vivo Neuronal Aggregate and Organelle Extrusion in Large Exopher Vesicles in C. elegans

Published on: September 18, 2020

Author Spotlight: Advanced Techniques for Visualizing Endogenous Axonal Transport Dynamics

Author Spotlight: Advanced Techniques for Visualizing Endogenous Axonal Transport Dynamics

Published on: February 16, 2024

Videos de Conceptos Relacionados

Maturation of Endosomes

Maturation of Endosomes

The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
Changes in location
The maturing endosome moves along microtubules from the periphery of the cell towards the perinuclear region. This movement of the...

The Early Endosome: Endocytosis of Transferrin

The Early Endosome: Endocytosis of Transferrin

Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...

Export of Misfolded Proteins out of the ER

Export of Misfolded Proteins out of the ER

After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...

Recycling Endosomes and Transcytosis

Recycling Endosomes and Transcytosis

The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...

Post-translational Translocation of Proteins to the RER

Post-translational Translocation of Proteins to the RER

A sizable fraction of proteins destined for ER are first synthesized in the cell cytosol and then transported across the ER membrane–a process called post-translational translocation. Similar to cotranslationally translocated proteins, these proteins also use the Sec translocon complex to enter the ER lumen.
Targeting proteins to the ER
Hsp40 and Hsp70 chaperone molecules bind the translated proteins in the cytosol to prevent their folding. The chaperone binding helps to keep the signal...

Introduction to Membrane Traffic

Introduction to Membrane Traffic

The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...