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Las bacterias intracelulares encuentran el movimiento correcto.
Edith Gouin1, Juan-Jose Quereda, Juan-Jose Quereda Torres1
1Unité des Interactions bactéries-Cellules, Institut Pasteur, 75015 Paris, France; Inserm U604, 75015 Paris, France; INRA USC2020, 75015 Paris, France.
Cell
|April 11, 2015
Resumen
Las bacterias de Burkholderia usan proteínas de polimerización de actina para moverse dentro de las células huésped. Estas bacterias imitan las proteínas del huésped para explotar la dinámica de la actina celular para la diseminación.
Área de la Ciencia:
- Microbiología Microbiología.
- Biología celular Biología celular.
- La patogénesis bacteriana es la patogénesis bacteriana.
Sus antecedentes:
- Las bacterias Burkholderia pseudomallei y Burkholderia mallei son bacterias Gram negativas.
- La motilidad basada en la actina es un mecanismo utilizado por algunos patógenos para la invasión y propagación de las células huésped.
- Las proteínas Ena/Vasp son reguladores clave de la polimerización de la actina en las células eucariotas.
Objetivo del estudio:
- Investigar el mecanismo por el cual las bacterias Burkholderia logran motilidad basada en actina.
- Para identificar las proteínas bacterianas involucradas en la polimerización de la actina.
- Para entender cómo estas bacterias secuestran la maquinaria de la célula huésped para la diseminación.
Principales métodos:
- Análisis de cultivo bacteriano y expresión de proteínas.
- Imágenes de células vivas para observar la motilidad bacteriana.
- Ensayos bioquímicos para estudiar las interacciones de las proteínas y la polimerización de la actina.
Principales resultados:
- Benanti y otros. proteínas bacterianas identificadas expresadas por Burkholderia pseudomallei y Burkholderia mallei.
- Se descubrió que estas proteínas bacterianas imitan la función de las proteínas de la familia Ena/Vasp.
- La imitación permitió a las bacterias inducir la polimerización de la actina, lo que lleva a la motilidad basada en la actina.
Conclusiones:
- Las bacterias Burkholderia poseen un mecanismo para inducir la polimerización de actina mediante la expresión de proteínas similares a Ena/Vasp.
- Esta estrategia bacteriana facilita la diseminación intracelular e intercelular dentro del huésped.
- Comprender este mecanismo ofrece información sobre la patogénesis bacteriana y posibles objetivos terapéuticos.
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