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El gen activador de la recombinación V(D) J, RAG-1

D G Schatz1, M A Oettinger, D Baltimore

  • 1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142.

Cell
|December 22, 1989
PubMed
Resumen

Los investigadores identificaron el gen RAG-1, crucial para la recombinación de V (D) J en las células inmunes. Este gen es este gen.

Área de la Ciencia:

  • Inmunología Inmunología.
  • Biología Molecular Biología Molecular
  • Genética La genética.

Sus antecedentes:

  • La recombinación J es esencial para la inmunidad adaptativa.
  • El papel del gen RAG-1 en la iniciación de este proceso está siendo investigado.

Objetivo del estudio:

  • Para aislar y caracterizar el locus genómico RAG-1.
  • Determinar la función y el patrón de expresión de RAG-1.

Principales métodos:

  • Transfecciones genómicas en serie de ADN etiquetado con oligonucleótidos.
  • Caminata genómica y análisis de manchas del sur.
  • Secuenciación de nucleótidos de clones de ADNc RAG-1.

Principales resultados:

  • Se aisló el locus genómico RAG-1, que abarca 55 kb.

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  • Una sonda RAG-1 detectó una sola especie de ARNm en los tipos de células relevantes.
  • Los clones RAG-1 eran biológicamente activos en las células 3T3 de NIH.
  • El cDNA RAG-1 humano y del ratón predice proteínas de 119 kd altamente conservadas.

    • Conclusiones:

    • La expresión de RAG-1 se correlaciona con la actividad de la recombinasa V(D) J.
    • RAG-1 se conserva en todas las especies con recombinación V(D) J.
    • RAG-1 puede codificar directamente o activar indirectamente la recombinación V(D) J.