Video Experimental Relacionado

Updated: Feb 7, 2026

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Measurement of Microtubule Dynamics by Spinning Disk Microscopy in Monopolar Mitotic Spindles

Published on: November 15, 2019

Spastin y ESCRT-III coordinan el desmontaje del huso mitótico y el sellado de la envolvente nuclear

Marina Vietri¹, Kay O Schink¹, Coen Campsteijn¹

¹1] Centre for Cancer Biomedicine, Faculty of Medicine, University of Oslo, Montebello, N-0379 Oslo, Norway [2] Department of Molecular Cell Biology, Institute for Cancer Research, Oslo University Hospital, Montebello, N-0379 Oslo, Norway.

|June 5, 2015

Resumen

El complejo de clasificación endosómica requerido para la maquinaria de transporte (ESCRT-III) es crucial para sellar la envoltura nuclear durante la división celular. Este proceso coordina el sellado de la envoltura nuclear con el desmontaje de los microtúbulos de huso, asegurando la integridad del genoma.

Área de la Ciencia:

Biología celular Biología celular.
Biología Molecular Biología Molecular
Genética La genética.

Sus antecedentes:

La división celular de los metazoos implica la ruptura y el reensamblaje de la envoltura nuclear.
Los mecanismos que coordinan el sellado de la envoltura nuclear y el desmontaje del husillo siguen siendo en gran parte desconocidos.

Objetivo del estudio:

Investigar el papel de la ESCRT-III en el reensamblaje de la envoltura nuclear durante la salida mitótica.
Para dilucidar la coordinación entre el sellado de la envoltura nuclear y el desmontaje del microtúbulo del huso.

Principales métodos:

Microscopía de inmunofluorescencia para visualizar la localización del ESCRT-III.
La manipulación genética para interrumpir los componentes del ESCRT-III y el spastin.
Análisis de la integridad de la envoltura nuclear y el daño del ADN.

Principales resultados:

El ESCRT-III es reclutado para la envoltura nuclear de reensamblaje durante la anáfase tardía.
CHMP7 recluta a ESCRT-III/VPS4 para las intersecciones de envoltura nuclear y microtúbulos.
IST1 recluta espastina para cortar los microtúbulos, lo que facilita el desmontaje del husillo.
La interrupción de la espastina o ESCRT-III perjudica el sellado de la envoltura nuclear y el desmontaje del husillo, lo que lleva a daños en el ADN.

Conclusiones:

ESCRT-III, VPS4 y spastin cooperan para coordinar el sellado de la envoltura nuclear y el desmontaje del husillo.
Esta coordinación es esencial para mantener la integridad nuclear y salvaguardar el genoma.
El mecanismo comparte paralelismos con los procesos de absisión celular.

Más Videos Relacionados

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets

Published on: August 13, 2016

Videos de Conceptos Relacionados

The Mitotic Spindle

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...

Coordination Number and Geometry

Coordination Number and Geometry

For transition metal complexes, the coordination number determines the geometry around the central metal ion. Table 1 compares coordination numbers to molecular geometry. The most common structures of the complexes in coordination compounds are octahedral, tetrahedral, and square planar.

Coordination Compounds and Nomenclature

Coordination Compounds and Nomenclature

In most main group element compounds, the valence electrons of the isolated atoms combine to form chemical bonds that satisfy the octet rule. For instance, the four valence electrons of carbon overlap with electrons from four hydrogen atoms to form CH4. The one valence electron leaves sodium and adds to the seven valence electrons of chlorine to form the ionic formula unit NaCl (Figure 1a). Transition metals do not normally bond in this fashion. They primarily form coordinate covalent bonds, a...

Spindle Assembly

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...

Lattice Centering and Coordination Number

Lattice Centering and Coordination Number

The structure of a crystalline solid, whether a metal or not, is best described by considering its simplest repeating unit, which is referred to as its unit cell. The unit cell consists of lattice points that represent the locations of atoms or ions. The entire structure then consists of this unit cell repeating in three dimensions. The three different types of unit cells present in the cubic lattice are illustrated in Figure 1.
Types of Unit Cells
Imagine taking a large number of identical...

Disassembly of Intermediate Filaments

Disassembly of Intermediate Filaments

Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...