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La unión competitiva de péptidos antagónicos ajusta finamente el patrón estomático

Jin Suk Lee¹, Marketa Hnilova², Michal Maes³

¹1] Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195, USA [2] Department of Biology, University of Washington, Seattle, Washington 98195, USA.

|June 18, 2015

Resumen

Los factores de patronaje epidérmico vegetal (EPF) controlan el desarrollo estomático. El péptido del estómago promueve la formación de estomas al unirse de manera competitiva a los receptores de ERECTA (ER), interfiriendo con la inhibición de EPF2.

Área de la Ciencia:

Biología vegetal Biología vegetal
La señalización molecular.
Biología del desarrollo Biología del desarrollo.

Sus antecedentes:

Los estomas de las plantas son cruciales para el intercambio de gases y se desarrollan en base a señales posicionales.
Los factores de patronaje epidérmico (EPF) son péptidos secretados que regulan el desarrollo estomático.
Los mecanismos moleculares precisos por los cuales los EPF orquestan la formación de patrones estomales no se comprenden completamente.

Objetivo del estudio:

Aclarar el mecanismo molecular por el cual el péptido Stomagen influye en el desarrollo estomático.
Para investigar la interacción entre Stomagen, EPF2 y las quinasas receptoras de la familia ERECTA (ER).
Para entender cómo estas interacciones modulan el patrón estomático en Arabidopsis.

Principales métodos:

Interacciones investigadas de péptido-receptor utilizando ensayos de unión.
Analizó el efecto de Stomagen y EPF2 en el desarrollo estomático en Arabidopsis.
Examinó el estado de fosforilación de los componentes de señalización aguas abajo en respuesta a los tratamientos con péptidos.

Principales resultados:

Stomagen promueve el desarrollo estomático mediante la interacción con las quinasas receptoras de la familia ERECTA (ER).
El estómago inhibe competitivamente la unión del FACTOR 2 de modelado epidérmico (EPF2) a ER y su co-receptor.
La aplicación de EPF2 indujo una rápida fosforilación de los componentes de señalización aguas abajo, mientras que Stomagen no lo hizo.

Conclusiones:

Stomagen actúa como un agonista, mientras que EPF2 actúa como un antagonista en el desarrollo estomático.
La unión competitiva de Stomagen y EPF2 a los receptores ER ajusta el patrón estomático.
Este estudio revela un mecanismo molecular de cómo las señales inductivas e inhibidoras regulan el patrón de los tejidos en las plantas.

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