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Los inhibidores G1/S y la salida del ciclo celular de control del complejo SWI/SNF durante la diferenciación muscular

Suzan Ruijtenberg¹, Sander van den Heuvel¹

¹Developmental Biology, Department of Biology, Faculty of Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, the Netherlands.

|July 7, 2015

Resumen

La salida del ciclo celular durante la diferenciación implica mecanismos redundantes. El complejo SWI / SNF y los inhibidores G1 / S proporcionan vías alternativas para detener la división celular, evitando un fenotipo tumoral.

Área de la Ciencia:

Biología del desarrollo Biología del desarrollo.
Biología celular Biología celular.
Genética La genética.

Sus antecedentes:

La salida del ciclo celular es vital para el desarrollo del organismo, el mantenimiento de los tejidos y la prevención del crecimiento celular incontrolado.
Comprender los mecanismos moleculares que gobiernan la detención del ciclo celular durante la diferenciación es crucial.

Objetivo del estudio:

Para investigar los mecanismos in vivo de salida del ciclo celular durante la diferenciación.
Para identificar los reguladores redundantes de la división celular detención.

Principales métodos:

Desarrollo de un sistema de nocaut condicional y rastreo de linaje en Caenorhabditis elegans.
La inactivación de genes específicos del linaje y el cribado genético.
Análisis del complejo de remodelación de la cromatina SWI/SNF y los inhibidores G1/S.

Principales resultados:

Se encontró una redundancia funcional extensa entre los inhibidores del ciclo celular y el complejo SWI / SNF.
La inactivación simultánea de los inhibidores SWI/SNF y G1/S condujo a una proliferación continua y a un fenotipo tumoral en C. elegans.
SWI / SNF actúa con hlh-1 MyoD, antagoniza la represión de Polycomb y suprime la transcripción de Cyclin E de cye-1.

Conclusiones:

Los inhibidores SWI/SNF y G1/S ofrecen rutas alternativas para la detención del ciclo celular durante la diferenciación terminal.
Estos hallazgos proporcionan información sobre las mutaciones genéticas SWI / SNF en los cánceres humanos.

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