Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

823
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
823
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

1.2K
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
1.2K
Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

12.5K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
12.5K
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview01:26

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview

4.1K
Nitrous acid and nitric acids are two types of acids containing nitrogen, among which nitrous acid is weaker than nitric acid. Nitrous acid with a pKa value of 3.37 ionizes in water to give a nitrite ion and the hydronium ion.
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
4.1K
Preparation of Nitriles01:12

Preparation of Nitriles

2.8K
One of the common methods to prepare nitriles is the dehydration of amides. This method requires strong dehydrating agents like phosphorous pentoxide or boiling acetic anhydride for converting amides to nitriles. Another reagent namely, thionyl chloride also accomplishes the dehydration of amides, where amide acts as a nucleophile. The first step of the mechanism involves the nucleophilic attack by the amide on the thionyl chloride to form an intermediate. In the next step, the electron pairs...
2.8K
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism01:37

1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism

5.3K
Nitrous acid is a relatively weak and unstable acid prepared in situ by the reaction of sodium nitrite and cold, dilute hydrochloric acid. In an acidic solution, the nitrous acid undergoes protonation when it loses water to form a nitrosonium ion—an electrophile. Nitrous acid reacts with primary amines to give diazonium salts. The reaction is called diazotization of primary amines.
5.3K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Qualitative profiling of the gut-specific chlamydial population in Ixodes ricinus ticks.

Ticks and tick-borne diseases·2026
Same author

Effects of Increasing the Concentration of Dialysate Magnesium on Cardiovascular Health: A Narrative Review.

Canadian journal of kidney health and disease·2026
Same author

From "synthetic" to defined microbial communities for clearer terminology.

Nature communications·2026
Same author

Mixed feeding and mode of birth modulate the effects of a hydrolyzed synbiotic formula on the gut microbiome in infants at risk of atopic disease.

Clinical nutrition ESPEN·2026
Same author

Immune-mediated microbial interference governs <i>Borrelia</i> colonization of the tick gut.

iScience·2026
Same author

Benchmarking of shotgun sequencing depth reveals the potential and limitations of shallow metagenomics and strain-level analysis.

Nature microbiology·2026

Video Experimental Relacionado

Updated: Apr 6, 2026

Electrochemically and Bioelectrochemically Induced Ammonium Recovery
09:50

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Published on: January 22, 2015

13.3K

El cianato como fuente de energía para los nitrificadores

Marton Palatinszky1, Craig Herbold1, Nico Jehmlich2

  • 1Department of Microbiology and Ecosystem Science, Division of Microbial Ecology, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.

Nature
|July 30, 2015
PubMed
Resumen

Este estudio revela que el thaumarchaeote Nitrososphaera gargensis oxidante de amoníaco puede crecer utilizando el cianato como su única fuente de energía, una capacidad metabólica previamente desconocida. Este hallazgo destaca el cianato

Más Videos Relacionados

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.8K
Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

8.8K

Videos de Experimentos Relacionados

Last Updated: Apr 6, 2026

Electrochemically and Bioelectrochemically Induced Ammonium Recovery
09:50

Electrochemically and Bioelectrochemically Induced Ammonium Recovery

Published on: January 22, 2015

13.3K
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.8K
Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors
07:59

Estimating Sediment Denitrification Rates Using Cores and N2O Microsensors

Published on: December 6, 2018

8.8K

Área de la Ciencia:

  • Microbiología
  • Ciencias del medio ambiente
  • Biogeoquímica

Sus antecedentes:

  • Los microorganismos oxidantes de amoníaco y nitrito son cruciales para el ciclo global del nitrógeno.
  • Tradicionalmente, el amoníaco y la urea son las únicas fuentes de energía conocidas para el crecimiento aeróbico de bacterias y arqueas oxidantes de amoníaco.

Objetivo del estudio:

  • Investigar el potencial de crecimiento aeróbico de los microorganismos oxidantes de amoníaco utilizando nuevas fuentes de energía.
  • Identificar y caracterizar las vías metabólicas implicadas en la utilización de estas nuevas fuentes de energía.

Principales métodos:

  • Aislamiento y cultivo de Nitrososphaera gargensis utilizando cianato como única fuente de energía.
  • Ensayos enzimáticos para determinar las vías de conversión del cianato.
  • Cribado metagenómico de muestras ambientales para evaluar la prevalencia de genes relevantes.

Principales resultados:

  • El crecimiento aeróbico de Nitrososphaera gargensis se logró utilizando cianato como única fuente de energía y reductor.
  • Se identificó una enzima cianasa en N. gargensis, responsable de la conversión de cianato en amonio.
  • Los experimentos de cocultivo demostraron la alimentación recíproca entre los microorganismos oxidantes de amoníaco y los oxidantes de nitrito utilizando cianato.

Conclusiones:

  • Nitrososphaera gargensis exhibe una versatilidad metabólica inesperada al utilizar cianato para el crecimiento.
  • El cianato representa un compuesto previamente no reconocido pero importante en el ciclo del nitrógeno ambiental.
  • La amplia distribución de los genes de la cianasa sugiere un papel significativo para el metabolismo del cianato en diversos ecosistemas.