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Arquitectura del canal Piezo1 mecanicamente sensible de los mamíferos

Jingpeng Ge^1,2, Wanqiu Li², Qiancheng Zhao^1,3

¹Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences or Medicine, Tsinghua University, Beijing 100084, China.

|September 22, 2015

Resumen

Los investigadores revelaron la estructura de Piezo1, un canal de iones mecanicamente sensible. Este descubrimiento ofrece información sobre cómo los canales Piezo1 perciben las fuerzas mecánicas y se abren para permitir el flujo de iones.

Área de la Ciencia:

La biofísica
Biología molecular
Biología estructural

Sus antecedentes:

Las piezo proteínas son canales iónicos mecano-sensibles críticos.
Su organización estructural y mecanismos de puertas permanecen en gran medida sin caracterizar.

Objetivo del estudio:

Para determinar la estructura de alta resolución del canal Piezo1 del ratón de longitud completa.
Para aclarar la base estructural para la mecanotransducción Piezo1.

Principales métodos:

Se empleó la crio-microscopía electrónica (crio-EM).
La estructura del ratón Piezo1 de cuerpo entero (2.547 aminoácidos) se determinó a una resolución de 4,8 Å.

Principales resultados:

Piezo1 forma un complejo grande, trímero y parecido a una hélice (~ 900 kDa).
La estructura revela distintas cuchillas extracelulares, una tapa central y una región transmembrana con 14 segmentos por subunidad.
Un módulo de poro central está encerrado por alas periféricas, lo que sugiere un mecanismo de bloqueo de fuerza.

Conclusiones:

La estructura determinada proporciona un marco para la comprensión de la función Piezo1.
La arquitectura sugiere que los dominios periféricos actúan como sensores de fuerza para abrir el poro central de iones.

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