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Conversión de alcoholes en aminas enantiopuras a través de cascadas de préstamo de hidrógeno de doble enzima

Francesco G Mutti¹, Tanja Knaus², Nigel S Scrutton²

¹School of Chemistry, University of Manchester, Manchester Institute of Biotechnology, Manchester M1 7DN, UK. Manchester Institute of Biotechnology, Faculty of Life Sciences, University of Manchester, Manchester M1 7DN, UK. nicholas.turner@manchester.ac.uk f.mutti@uva.nl.

Science (New York, N.Y.)

|September 26, 2015

Resumen

Este estudio introduce un método biocatalítico que utiliza dos enzimas para la producción eficiente y ecológica de aminas enantiopuras a partir de alcoholes. El proceso logra altos rendimientos y pureza, ofreciendo una ruta sostenible para la síntesis de aminas quirales.

Área de la Ciencia:

Biocatálisis
Síntesis orgánica
Química ecológica

Sus antecedentes:

Las aminas quirales son bloques vitales en la síntesis química industrial.
Los métodos actuales para producir aminas enantiopuras pueden ser ineficientes o exigentes desde el punto de vista ambiental.

Objetivo del estudio:

Desarrollar un método biocatalítico eficiente y respetuoso con el medio ambiente para la producción de aminas enantiopuras.
Para utilizar una estrategia de aminación de préstamo de hidrógeno con un sistema de doble enzima.

Principales métodos:

Se empleó una cascada enzimática en tándem que combina alcohol deshidrogenasa y amina deshidrogenasa de ingeniería.
Utilizó una gama estructuralmente diversa de alcoholes aromáticos y alifáticos como sustratos.
Se obtiene nitrógeno del amonio, creando un sistema autosuficiente de oxidación.

Principales resultados:

Se obtienen altas conversiones (hasta el 99%) para la aminación primaria de alcohol.
Se obtiene un excelente exceso enantiomérico (hasta el 99%) para las aminas quirales producidas.
Se ha demostrado una alta eficiencia atómica con agua como único subproducto.

Conclusiones:

El sistema biocatalítico de doble enzima presentado proporciona una vía sostenible y eficiente para la producción de aminas enantiopuras.
Este método ofrece una alternativa ecológica para la síntesis de aminointermedios quirales clave a escala industrial.

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