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Disfunción de los glóbulos rojos inducida por una dieta alta en grasas: implicaciones potenciales para la

Dusten Unruh¹, Ramprasad Srinivasan¹, Tyler Benson¹

¹From Hematology/Oncology, Department of Internal Medicine, College of Medicine, University of Cincinnati, OH (D.U., R.S., R.K., R.S., V.B., M.P., R.S.F., V.Y.B.); Vascular Biology Center, Georgia Regents University, Augusta, GA (T.B., S.H., T.C., D.J.F., N.L.W.); Cardiovascular Diseases, Department of Internal Medicine, College of Medicine, University of Cincinnati, OH (D.C., N.B., W.T., D.M.); Department of Nutritional Sciences, College of Allied Health Sciences, University of Cincinnati, OH (D.C., A.P.); Department of Pathology, College of Medicine, University of Cincinnati, OH (D.Y.H., W.S.D.); Biomedical Informatics and Developmental Biology / Department of Pediatrics, College of Medicine, University of Cincinnati and Cincinnati Children's Hospital and Medical Center, OH (B.J.A.); Experimental Hematology / Department of Pediatrics, College of Medicine, University of Cincinnati and Cincinnati Children's Hospital and Medical Center, OH (T.K.); Hemoshear LLC, Charlottesville, VA (D.M.); and Department of Emergency Medicine, University of Texas Southwestern Medical Center, Dallas (A.B.).

|October 16, 2015

Resumen

Las dietas altas en grasas inducen la disfunción de los glóbulos rojos, aumentando la unión de quimiocinas e interacciones inflamatorias. Esta disfunción de los glóbulos rojos puede contribuir al desarrollo de aterosclerosis en la obesidad.

Área de la Ciencia:

Ciencias cardiovasculares
Hematología
Investigación de las enfermedades metabólicas

Sus antecedentes:

Se sabe que las dietas altas en grasas (HFD) promueven la disfunción endotelial y la activación de los monocitos, lo que contribuye a la aterosclerosis en la obesidad.
Los glóbulos rojos (glóbulos rojos) pueden actuar como reservorios de quimiocinas a través del receptor de antígeno de Duffy para las quimiocinas (DARC).

Objetivo del estudio:

Para investigar si el HFD induce disfunción en los glóbulos rojos.
Explorar el papel de la disfunción de los glóbulos rojos en la obesidad y la aterosclerosis inducidas por la dieta.

Principales métodos:

Los ratones fueron alimentados con un 60% de HFD durante 12 semanas.
Niveles evaluados de proteína quimioatractiva monocítica-1 (MCP-1) y KC unida a las glóbulos rojos.
Se analizó el colesterol de la membrana de los glóbulos rojos, la externalización de fosfatidilserina y la absorción del bazo.
Interacciones evaluadas de glóbulos rojos y macrófagos y activación endotelial in vitro y ex vivo.

Principales resultados:

La HFD aumentó significativamente el MCP- 1 ligado a los glóbulos rojos y el KC, dependiendo del DARC.
Los glóbulos rojos de los ratones alimentados con HFD mostraron un aumento del colesterol en la membrana y la externalización de fosfatidilserina.
Los glóbulos rojos inducidos por HFD mostraron una mayor absorción del bazo y promovieron la adhesión de los macrófagos al endotelio.
Estos efectos fueron más pronunciados en ratones DARC.

Conclusiones:

La disfunción de los glóbulos rojos ocurre temprano en la obesidad inducida por la dieta, similar a la disfunción endotelial.
Los glóbulos rojos disfuncionales pueden mediar la aterosclerosis promoviendo las interacciones inflamatorias y la activación endotelial.
Estos hallazgos destacan un papel novedoso para los glóbulos rojos en la patogénesis de la aterosclerosis relacionada con la obesidad.

Palabras clave:

la aterosclerosis Los eritrocitos Leucócitos Obesidad

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