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Identificación de un factor de unión al ADN implicado en el control del ciclo celular del gen HO de la levadura
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Cell
|April 7, 1989
Resumen
La regulación del ciclo celular del gen HO implica el factor de caja del ciclo celular (CCBF). Los genes SWI4 y SWI6 son esenciales para la actividad CCBF y la expresión génica HO durante la fase G1.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética de la levadura Genética de la levadura
- El Reglamento del Ciclo Celular.
Sus antecedentes:
- La transcripción del gen HO está fuertemente regulada por múltiples factores de acción trans.
- Los reguladores específicos y los elementos de la caja del ciclo celular (CCB) controlan la expresión de HO durante la fase G1 tardía.
Objetivo del estudio:
- Para identificar el factor de unión a los elementos de caja del ciclo celular.
- Determinar el papel de los genes SWI en la formación del complejo promotor CCBF y la expresión génica HO.
Principales métodos:
- Pruebas de retardo de gel para detectar las interacciones proteína-ADN.
- La huella de DNase para mapear los sitios de unión del factor.
- Pruebas in vitro e in vivo para evaluar la expresión génica.
Principales resultados:
- Se identificó un nuevo factor, el factor de caja del ciclo celular (CCBF), que se une a los elementos CCB.
- Los genes SWI4 y SWI6 son necesarios para la formación del complejo promotor de CCBF in vitro.
- SWI4 y SWI6 son los únicos genes SWI necesarios para la expresión génica HO mediada por CCB in vivo.
Conclusiones:
- CCBF es un regulador clave de la transcripción del gen HO dependiente del ciclo celular.
- SWI4 y SWI6 desempeñan funciones específicas y cruciales en la mediación de esta regulación del ciclo celular.
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