Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.6K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.6K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

3.6K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.6K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

4.2K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
4.2K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

7.0K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
7.0K
Adult Stem Cells01:33

Adult Stem Cells

34.3K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
34.3K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Exceptional Longevity Modifying Allele APOE2 Promotes DNA Signaling Pathways Resisting Cellular Senescence in Human Neurons.

Aging cell·2026
Same author

Conserved role of Atx2 in JNK pathway activation.

Cell death & disease·2026
Same author

Maladaptive Inflammatory Signaling in Old Mice Impairs Colonic Regeneration by Promoting a Sustained Fetal-Like Epithelial State.

Aging cell·2026
Same author

Toward actionable interventions in human aging (12th ARDD meeting, 2025).

Aging·2026
Same author

Advancing senescence translation through the Senotherapeutics Biomarker Consortium.

Nature aging·2026
Same author

CREB suppresses PGRP-SC2 to drive age-related immune senescence and gut dysbiosis in Drosophila.

Cell death discovery·2026

Video Experimental Relacionado

Updated: Mar 29, 2026

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

9.9K

La integración de la señal por Ca2+) regula la actividad de las células madre intestinales

Hansong Deng1, Akos A Gerencser1, Heinrich Jasper1

  • 1The Buck Institute for Research on Aging, 8001 Redwood Boulevard, Novato, California 94945, USA.

Nature
|December 4, 2015
PubMed
Resumen

El L-glutamato en la dieta aumenta la división de las células madre intestinales (CIE) y el crecimiento intestinal en Drosophila. La señalización del calcio (Ca2+), regulada por los receptores metabotrópicos del glutamato (mGluR), controla la proliferación de la ISC a través de la calcineurina y el Crtc.

Más Videos Relacionados

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

74.2K
Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
11:33

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

9.6K

Videos de Experimentos Relacionados

Last Updated: Mar 29, 2026

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ
09:34

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

9.9K
Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
07:42

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Published on: July 13, 2016

74.2K
Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
11:33

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

9.6K

Área de la Ciencia:

  • Biología de las células madre
  • Señales de calcio
  • Organismo modelo de Drosophila melanogaster

Sus antecedentes:

  • Las células madre somáticas mantienen la homeostasis del tejido a través de la proliferación y la diferenciación dinámicas.
  • El estrés y las señales metabólicas influyen en el comportamiento de las células madre.
  • Las células madre intestinales (CIE) son cruciales para la salud y la regeneración intestinales.

Objetivo del estudio:

  • Identificar los reguladores clave de la actividad de las células madre intestinales (CIE) en Drosophila.
  • Para aclarar el papel de la señalización del calcio (Ca2+) en la proliferación de las CSI.
  • Para entender cómo las señales dietéticas modulan el comportamiento ISC.

Principales métodos:

  • Investigó Drosophila melanogaster como un sistema modelo.
  • Utilizó el L-glutamato de la dieta como un estímulo.
  • Se examinó el papel de los receptores metabotrópicos del glutamato (mGluR) en las CSI.
  • Oscilaciones y concentraciones citosólicas de Ca2+.
  • Se analizó la participación de la calcineurina y el coactivador transcripcional regulado por CREB (Crtc).

Principales resultados:

  • El L-glutamato dietético estimula la división de la célula intestinal y el crecimiento intestinal.
  • Los receptores metabotrópicos del glutamato (mGluR) son esenciales para esta respuesta en las CSI.
  • La activación de mGluR modula las oscilaciones citosólicas de Ca2+, lo que lleva a niveles sostenidos de Ca2+.
  • El alto contenido citosólico de Ca2+ induce la proliferación de ISC a través de la calcineurina y el Crtc.
  • Las CSI exhiben transiciones reversibles entre los estados de oscilación de Ca2+, lo que refleja los modos de proliferación.

Conclusiones:

  • La señalización de calcio (Ca2+) es un regulador central de la actividad de las células madre intestinales (CIE) en Drosophila.
  • La regulación dinámica del Ca2+ intracelular permite a las ISC integrar diversas señales y adaptarse a la proliferación.
  • Este mecanismo permite que los CSI ajusten la actividad proliferativa de acuerdo con las necesidades de los tejidos en respuesta a estímulos dietéticos y de estrés.