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Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
Una putativa proteína quinasa supera la detención del ciclo celular inducida por las feromonas en S. cerevisiae
W E Courchesne1, R Kunisawa, J Thorner
1Department of Biochemistry, University of California, Berkeley 94720.
Cell
|September 22, 1989
Resumen
La sobreexpresión del gen KSS1 ayuda a las células de levadura a recuperarse de la detención del ciclo celular inducida por feromonas de apareamiento. Esta función del gen KSS1 es independiente de otras vías de recuperación conocidas e involucra al gen WHI1.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Regulación del ciclo celular Reglamento del ciclo celular.
- Genética de la levadura Genética de la levadura
Sus antecedentes:
- Las feromonas de apareamiento de la levadura inducen una detención del ciclo celular G1.
- La recuperación de esta detención requiere procesos celulares específicos, incluidos los que involucran el producto del gen SST2 y la cola citoplasmática del receptor del factor alfa.
Objetivo del estudio:
- Para investigar el papel del gen KSS1 en la recuperación del ciclo celular de la levadura después de una parada inducida por feromonas.
- Identificar nuevas vías involucradas en la desensibilización a las feromonas de apareamiento.
Principales métodos:
- Análisis genético de mutantes de levadura (células MATa con mutación sst2).
- Estudios de la sobreexpresión genética (gen KSS1).
- Análisis de las variantes del receptor del factor alfa que carecen de la cola citoplasmática.
- Investigando las interacciones genéticas (KSS1 y WHI1).
Principales resultados:
- La sobreexpresión del gen KSS1 suprime el defecto de recuperación en las células mutantes sst2.
- La sobreexpresión de KSS1 también rescata el defecto en células con un receptor truncado del factor alfa.
- KSS1 actúa independientemente de SST2 y la cola del receptor para promover la desensibilización.
- La actividad de recuperación de KSS1 depende del gen WHI1, lo que sugiere una vía compartida.
Conclusiones:
- KSS1 codifica una proteína quinasa que representa una tercera vía independiente para la desensibilización y la recuperación de la detención de las feromonas.
- KSS1 y WHI1 (un homólogo de la ciclina) probablemente funcionen juntos en una vía común de control del crecimiento.
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