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SPT15, el gen que codifica el factor de unión TATA de la levadura TFIID, se requiere para la iniciación normal de la

D M Eisenmann¹, C Dollard, F Winston

¹Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.

|September 22, 1989

Resumen

Se descubrió que las mutaciones en el gen SPT15, que codifica el factor de unión TATA TFIID, alteran la transcripción génica. Este factor de transcripción esencial es crucial para el crecimiento, el apareamiento y la esporulación de las levaduras, afectando a numerosos genes.

Área de la Ciencia:

Biología Molecular Biología Molecular
Genética La genética.
Biología de la levadura Biología de la levadura.

Sus antecedentes:

Investigando los mecanismos de regulación genética en Saccharomyces cerevisiae.
Comprender el papel de los factores de transcripción en el control de la expresión génica.
El gen SPT15 fue previamente identificado en pantallas supresoras para la transcripción genética alterada.

Objetivo del estudio:

Para identificar y caracterizar el gen SPT15.
Para determinar la función del producto del gen SPT15 en la iniciación de la transcripción.
Evaluar la esencialidad y los efectos pleiotrópicos de las mutaciones de SPT15.

Principales métodos:

Aislamiento de mutaciones SPT15 como supresores de mutaciones de inserción.
La clonación molecular y el análisis genético del gen SPT15.
Análisis fenotípico de cepas mutantes spt15, incluidos ensayos de crecimiento, apareamiento y esporulación.

Principales resultados:

El gen SPT15 codifica el factor de unión TATA TFIID.
Las alteraciones en el TFIID (a través de mutaciones spt15) afectan la iniciación de la transcripción in vivo.
TFIID es esencial para el crecimiento de la levadura; los mutantes de spt15 exhiben un crecimiento lento y defectos en el apareamiento y la esporulación.

Conclusiones:

TFIID es un factor de transcripción esencial para la viabilidad de Saccharomyces cerevisiae.
TFIID juega un papel crítico en la regulación de la iniciación de la transcripción in vivo.
Las mutaciones de SPT15 revelan funciones pleiotrópicas para TFIID más allá de la transcripción basal.

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