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Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
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Biología y Aplicaciones de los Sistemas CRISPR: Aprovechando la Caja de Herramientas de la Naturaleza para la
Addison V Wright1, James K Nuñez1, Jennifer A Doudna2
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.
Cell
|January 16, 2016
Resumen
Los sistemas CRISPR-Cas proporcionan inmunidad adaptativa en los microbios al recordar y destruir el ADN extraño. Estos sistemas son ahora herramientas poderosas para la edición precisa del genoma en muchos organismos.
Área de la Ciencia:
- Microbiología
- Biología molecular
- La genética
Sus antecedentes:
- Las bacterias y las arqueas emplean mecanismos de defensa contra los elementos genéticos móviles y los virus.
- Los sistemas CRISPR-Cas ofrecen inmunidad adaptativa al retener la memoria genética de los invasores.
Objetivo del estudio:
- Revisar los avances recientes en la comprensión de los mecanismos de CRISPR-Cas.
- Para resaltar la aplicación de CRISPR-Cas en la manipulación del genoma.
Principales métodos:
- Revisión de la literatura actual sobre los sistemas CRISPR-Cas.
- Análisis de las interacciones de la proteína Cas con ácidos nucleicos extraños.
Principales resultados:
- Mecanismos detallados de orientación del ácido nucleico mediado por la proteína Cas.
- Diversas estrategias para la ingeniería genómica basada en CRISPR-Cas.
Conclusiones:
- Los sistemas CRISPR-Cas son mecanismos inmunes adaptativos versátiles.
- Estos sistemas se han adaptado con éxito para aplicaciones precisas de edición del genoma.
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