Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

The DNA Replication Fork01:02

The DNA Replication Fork

42.7K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
42.7K
The DNA Replication Fork01:02

The DNA Replication Fork

20.1K
20.1K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.5K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.5K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

2.5K
2.5K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.4K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.3K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Spatially resolved profiling of steroid nuclear receptors reveals a role for the disordered N-terminal domains in genome targeting and AP-1 interaction.

Genome research·2026
Same author

Selective autophagy fine-tunes plant immunity to promote cell survival during viral infection.

Science (New York, N.Y.)·2026
Same author

Engineering intrinsically disordered regions for guiding genome navigation.

Molecular cell·2026
Same author

Design principles of transcription factors with intrinsically disordered regions.

eLife·2025
Same author

Selective association of short tandem repeats with DNA-binding domains and intrinsically disordered regions of transcription factors.

Cell systems·2025
Same author

k-mer-based GWAS in a wheat collection reveals novel and diverse sources of powdery mildew resistance.

Genome biology·2025
Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026
Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026
Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026
Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026
Same journal

Retraction.

Science (New York, N.Y.)·2026
Same journal

Making time.

Science (New York, N.Y.)·2026
Ver todos los artículos relacionados

Video Experimental Relacionado

Updated: Mar 24, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

9.6K

Homeostasis de expresión durante la replicación del ADN

Yoav Voichek1, Raz Bar-Ziv1, Naama Barkai2

  • 1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.

Science (New York, N.Y.)
|March 5, 2016
PubMed
Resumen
Este resumen es generado por máquina.

La tasa de síntesis de ARN mensajero se amortigua durante la replicación del ADN en la levadura. Esta homeostasis de expresión se basa en la acetilación de la histona H3 K56, que reduce la eficiencia de la transcripción del ADN replicado.

Más Videos Relacionados

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.5K
Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights
08:30

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

Published on: December 22, 2023

3.6K

Videos de Experimentos Relacionados

Last Updated: Mar 24, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
09:14

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

9.6K
Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.5K
Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights
08:30

Author Spotlight: Visualizing Single-Stranded DNA During DNA Repair for Therapeutic Insights

Published on: December 22, 2023

3.6K

Área de la Ciencia:

  • Biología molecular
  • La epigenética
  • Genética de la levadura

Sus antecedentes:

  • La replicación del genoma aumenta la disponibilidad de la plantilla de ADN para la transcripción.
  • Los genes de replicación temprana se enfrentan a este aumento antes que los genes de replicación tardía, lo que plantea preguntas sobre la regulación de la expresión.

Objetivo del estudio:

  • Investigar cómo la síntesis del ARN mensajero (ARNm) se ve afectada por la replicación del ADN.
  • Identificar los mecanismos moleculares que mantienen la homeostasis de expresión durante la fase S.

Principales métodos:

  • Utilizó la levadura en ciernes como organismo modelo.
  • Niveles de expresión génica investigados durante la fase S.
  • Se examinó el papel de la acetilación de la histona H3 K56 y los factores asociados (Rtt109/Asf1).

Principales resultados:

  • La velocidad de síntesis de ARN mensajero se amortiguó contra los cambios en la dosis de genes durante la fase S.
  • Esta homeostasis de expresión es dependiente de la acetilación de la histona H3 K56 por Rtt109/Asf1.
  • La eliminación de estos factores o la mutación/deacetilación de H3K56 conduce a un aumento de la expresión génica proporcional al tiempo de replicación.

Conclusiones:

  • La acetilación de la histona H3 K56 en las histonas recién depositadas suprime la eficiencia de transcripción del ADN replicado.
  • Este mecanismo de acetilación contribuye a mantener la homeostasis de expresión durante la replicación del ADN.
  • El estudio proporciona información molecular sobre la estabilidad del genoma y la regulación de la expresión.