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Imágenes especulares de péptidos antimicrobianos proporcionan reflexiones sobre sus funciones y propiedades

Conan K Wang¹, Gordon J King¹, Anne C Conibear¹

¹Institute for Molecular Bioscience, The University of Queensland , Brisbane, Queensland 4072, Australia.

Journal of the American Chemical Society

|April 12, 2016

Resumen

Los investigadores sintetizaron péptidos antimicrobianos de hoja beta, incluido BTD-2, para estudiar sus interacciones en la membrana. La estructura de BTD-2 reveló un nuevo trímero y ensamblaje supramolecular, que vincula la actividad antimicrobiana con estructuras similares a las amiloides.

Área de la Ciencia:

La bioquímica
Biología estructural
Biología molecular

Sus antecedentes:

Los péptidos antimicrobianos (AMP) son cruciales para la inmunidad innata.
La relación estructura-función de las PMA de hojas beta sigue siendo incompleta.
Comprender los mecanismos de la PMA es vital para combatir la resistencia a los antibióticos.

Objetivo del estudio:

Sintetizar y caracterizar las formas enantioméricas de péptidos antimicrobianos de hoja beta (BTD-2, PG-1, PM-1).
Para aclarar el mecanismo de acción, centrándose en la interacción de la membrana.
Determinar la base estructural de la actividad antimicrobiana del BTD-2.

Principales métodos:

Síntesis de péptidos antimicrobianos enantioméricos.
Ensayos de actividad y de unión lipídica.
Cristalografía de rayos X (resolución de 1,45 Å) del BTD-2 racémico.

Principales resultados:

Los péptidos BTD-2, PG-1 y PM-1 exhiben una interacción de membrana independiente del receptor.
La estructura cristalina de BTD-2 revela un nuevo trímero antiparalelo.
BTD-2 forma una estructura supramolecular con un motivo de cierre estérico, parecido a las fibrillas amiloides.

Conclusiones:

El ensamblaje trímero y supramolecular de BTD-2 está vinculado a su función membrana-activa.
Este estudio proporciona evidencia estructural de un nuevo ensamblaje similar a las fibrillas en las AMP.
Se establece un vínculo estructural entre los péptidos antimicrobianos y los péptidos amiloides.

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