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Biosíntesis de un metalóforo de amplio espectro similar a las nicotianaminas en Staphylococcus aureus
Ghassan Ghssein1, Catherine Brutesco1, Laurent Ouerdane2
1Laboratoire de Bioénergétique Cellulaire, Institut de Biosciences et Biotechnology Aix-Marseille (BIAM), Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), 13108 Saint-Paul-lès-Durance, France. UMR 7265, Centre National de Recherche Scientifique, Saint-Paul-lès-Durance, France. Aix Marseille Université, Marseille, France.
Science (New York, N.Y.)
|May 28, 2016
Resumen
Staphylococcus aureus sintetiza un nuevo metalóforo, la estafilopina, esencial para la adquisición de metales vitales como el hierro y el zinc en los entornos de acogida. Esta vía es crucial para la supervivencia del patógeno y la infección.
Área de la Ciencia:
- Microbiología
- La bioquímica
- Biología de los patógenos
Sus antecedentes:
- La adquisición de metales microbianos es crítica en entornos de huéspedes limitados en nutrientes.
- Staphylococcus aureus requiere estrategias eficientes para limpiar los metales esenciales para la supervivencia y la virulencia.
Objetivo del estudio:
- Para aclarar la biosíntesis y la función de un nuevo metalóforo en Staphylococcus aureus.
- Identificar la base genética y el mecanismo enzimático para la producción de estafilopinas y el transporte de metales.
Principales métodos:
- Perfiles metabólicos para identificar los metabolitos microbianos.
- Mutagénesis dirigida para analizar la función genética.
- Ensayos bioquímicos para caracterizar las actividades enzimáticas.
Principales resultados:
- Descubrimiento de un operón de biosíntesis de estafilopina en Staphylococcus aureus.
- Identificación de tres enzimas clave: la histidina racemasa, la enzima similar a la nicotinamina sintetasa y la estafilopina deshidrogenasa.
- La estafilopina demostró que la adquisición de metales de amplio espectro (Ni, Co, Zn, Cu, Fe) es crucial para el crecimiento de patógenos.
Conclusiones:
- La estafilopina es un nuevo metalóforo de amplio espectro esencial para la homeostasis metálica de Staphylococcus aureus.
- La vía estafilopina se conserva en otros patógenos, destacando su importancia en las enfermedades infecciosas.
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