Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Microbiota of the Large Intestine01:27

Microbiota of the Large Intestine

1
The large intestine hosts the most densely populated microbial ecosystem in the human body. This complex community primarily consists of anaerobic bacteria, with Bacillota (formerly Firmicutes) and Bacteroidota (formerly Bacteroidetes) as the predominant groups. The distribution of these microbes varies along different sections of the large intestine, influenced by local environmental factors such as oxygen availability and nutrient composition.The cecum, located at the beginning of the large...
1
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

3.5K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
3.5K
Microbiota of the Stomach and Small Intestine01:27

Microbiota of the Stomach and Small Intestine

1
The human gastrointestinal (GI) tract is characterized by distinct physicochemical conditions that shape its microbial communities. Among these, the stomach presents a particularly challenging environment for microbial colonization due to its highly acidic pH, ranging from 1 to 3. This extreme acidity effectively limits microbial density. However, certain acid-tolerant microorganisms are capable of surviving in this niche. Notably, Helicobacter pylori can colonize the gastric mucosa,...
1
Functions of the Gut Microbiota01:18

Functions of the Gut Microbiota

1
The gut microbiota includes trillions of microorganisms that colonize the human gastrointestinal tract, including bacteria, archaea, viruses, and fungi. This complex ecosystem plays a critical role in maintaining intestinal and systemic health. Most of these microbes inhabit the large intestine, establishing a relatively stable and diverse community that contributes to gut homeostasis through various metabolic, immunological, and protective mechanisms.Dominant bacterial phyla, such as...
1
Bacterial Flora of the Large Intestine01:29

Bacterial Flora of the Large Intestine

1.9K
The gut microbiome is formed by a vast and diverse community of bacteria that colonizes our large intestine. These bacteria start residing in the gut from birth and continue diversifying throughout life, influenced by factors such as diet, lifestyle, and stress. The gut bacterial community also includes bacteria from food and those that enter the colon through the anus.
The normal gut flora of the colon plays a critical role in generating essential vitamins such as vitamins K, B5, and B7.
1.9K
Probiotics01:22

Probiotics

1
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
1

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

DNA damage induces p53-independent apoptosis through ribosome stalling.

Science (New York, N.Y.)·2024
Same author

Retrograde movements determine effective stem cell numbers in the intestine.

Nature·2022
Same author

Plasticity of Lgr5-Negative Cancer Cells Drives Metastasis in Colorectal Cancer.

Cell stem cell·2020
Same author

Chromatin restriction by the nucleosome remodeler Mi-2β and functional interplay with lineage-specific transcription regulators control B-cell differentiation.

Genes & development·2019
Same author

Live imaging of cell division in 3D stem-cell organoid cultures.

Methods in cell biology·2018
Same author

Integrative multi-omics analysis of intestinal organoid differentiation.

Molecular systems biology·2018

Video Experimental Relacionado

Updated: Mar 19, 2026

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
07:46

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Published on: October 11, 2022

4.1K

Las criptas del colon: refugio de los productos microbianos

Hugo J Snippert1

  • 1Molecular Cancer Research, Center for Molecular Medicine, University Medical Center Utrecht, 3584 CX Utrecht, the Netherlands.

Cell
|June 18, 2016
PubMed
Resumen

Los microbios intestinales producen metabolitos que pueden dañar las células madre del colon. Sin embargo, la estructura única de la cripta del colon impide que estas sustancias tóxicas lleguen y dañen las células madre.

Más Videos Relacionados

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
06:33

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Published on: March 6, 2015

36.1K
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
06:57

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

Published on: February 6, 2021

8.8K

Videos de Experimentos Relacionados

Last Updated: Mar 19, 2026

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo
07:46

Three-Dimensional Culture of Murine Colonic Crypts to Study Intestinal Stem Cell Function Ex Vivo

Published on: October 11, 2022

4.1K
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
06:33

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Published on: March 6, 2015

36.1K
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts
06:57

Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts

Published on: February 6, 2021

8.8K

Área de la Ciencia:

  • Gastroenterología y Biología Molecular
  • Biología de las células madre
  • Investigación del microbioma

Sus antecedentes:

  • Se cree que las criptas profundas del colon protegen las células madre de las toxinas.
  • Las células madre en el colon son cruciales para la regeneración del tejido y el mantenimiento de la salud intestinal.

Objetivo del estudio:

  • Investigar la interacción entre los metabolitos de la microbiota intestinal y la proliferación de las células madre del colon.
  • Comprender el papel de la arquitectura criptográfica en la protección de las células madre de los productos microbianos.

Principales métodos:

  • Utilizó técnicas avanzadas de imagen para visualizar criptas de colon y localización de células madre.
  • Se utilizó un análisis metabolómico para identificar los metabolitos microbianos clave.
  • Se han realizado experimentos in vitro e in vivo para evaluar el impacto de los metabolitos en la proliferación de células madre.

Principales resultados:

  • Se identificó un metabolito específico de la microbiota intestinal que inhibe la proliferación de células madre del colon.
  • Se demostró que la estructura física de las criptas del colon protege eficazmente a las células madre de este metabolito inhibidor.
  • Mostró el papel protector de la arquitectura criptográfica contra los subproductos microbianos potencialmente dañinos.

Conclusiones:

  • La arquitectura de la cripta del colon es un mecanismo de defensa crítico que protege a las células madre de los metabolitos microbianos intestinales inhibidores.
  • Esta protección estructural es esencial para mantener la función de las células madre y la homeostasis del tejido del colon.
  • Los hallazgos destacan la intrincada interacción entre el microbioma, la fisiología del huésped y la biología de las células madre en el colon.