Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

1.4K
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
1.4K
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

1.1K
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
1.1K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.9K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
1.9K
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

1.5K
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
1.5K
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.8K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.8K
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

4.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.7K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

Viral capsid delivery of cGAMP enhances STING-dependent antitumor immune response.

bioRxiv : the preprint server for biology·2026
Same author

Reliability-Aware Deep Learning Framework for Chemical Genotoxicity Prediction with Uncertainty Quantification.

Journal of chemical information and modeling·2026
Same author

Spectroscopic and Thermodynamic Investigation of U(IV)-Oxalate Complexation Equilibria: Effects of Ionic Strength and Temperature.

Inorganic chemistry·2026
Same author

Hydrocarbon Frameworks with Long-Range Order Synthesized via Olefin Metathesis.

Journal of the American Chemical Society·2026
Same author

Advancing chemical safety prediction: an integrated GNN framework with DFT-augmented cyclic compound solution.

Journal of cheminformatics·2026
Same author

Biosynthesis of Minimal C-Phycocyanin Chromophore Assemblies in <i>E. coli</i> Provides a Platform to Dissect Protein-Mediated Tuning of Exciton Transfer.

Journal of the American Chemical Society·2026

Video Experimental Relacionado

Updated: Mar 17, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.9K

(129) Xe Detección macromolecular basada en la relajación por RMN

Muller D Gomes1,2, Phuong Dao1,2, Keunhong Jeong1,2

  • 1Materials Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.

Journal of the American Chemical Society
|July 30, 2016
PubMed
Resumen

Este estudio introduce un nuevo sensor de relajación de RMN de xenón-129. Este método permite la detección sensible de moléculas grandes mediante la medición de los cambios en las tasas de relajación del xenón al unirse al objetivo.

Más Videos Relacionados

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

6.3K
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.3K

Videos de Experimentos Relacionados

Last Updated: Mar 17, 2026

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments
09:25

Author Spotlight: Exploring Intrinsically Disordered Protein Dynamics Through NMR Relaxation Experiments

Published on: November 1, 2024

2.9K
15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the &#181;s-ms Timescale
08:09

15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale

Published on: April 19, 2021

6.3K
Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins
07:24

Paramagnetic Relaxation Enhancement for Detecting and Characterizing Self-Associations of Intrinsically Disordered Proteins

Published on: September 23, 2021

2.3K

Área de la Ciencia:

  • Espectroscopia de resonancia magnética nuclear (RMN)
  • Detección de sustancias químicas
  • Análisis de las interacciones biomoleculares

Sus antecedentes:

  • La relajación por resonancia magnética nuclear (RMN) es sensible al movimiento molecular.
  • Las jaulas de criptofano pueden encapsular xenón (Xe) y ser funcionalizadas para el reconocimiento de objetivos.
  • Las aplicaciones de detección a menudo requieren estrategias de amplificación para una mayor sensibilidad.

Objetivo del estudio:

  • Desarrollar un enfoque de detección basado en la relajación de RMN de xenón-129 para detectar objetivos moleculares grandes.
  • Para utilizar la señal amplificada del xenón disuelto a granel para la detección sensible.
  • Para investigar la relación entre la fijación del objetivo, la caída del sensor y las tasas de relajación del xenón.

Principales métodos:

  • Se diseñó un sensor basado en criptofano funcionalizado con un elemento de interacción objetivo y un agente quelante de metal.
  • El sensor se utilizó para detectar objetivos biotinilados que se unen a la avidina.
  • Los cambios en la tasa de relajación de la RMN (129) Xe (T2) se midieron en el momento de la unión al objetivo.

Principales resultados:

  • La unión del objetivo al sensor alteró significativamente el tiempo de correlación rotacional del xenón encapsulado.
  • Esta alteración condujo a un aumento medible en la tasa de relajación de xenón en masa.
  • Tras la unión de un sensor que contenía biotina a la avidina a 1,5 μM, el xenón T2 libre se redujo en un factor de 4, lo que demuestra una detección sensible.

Conclusiones:

  • El enfoque de detección basado en la relajación de RMN (129) Xe desarrollado detecta efectivamente objetivos macromoleculares de gran tamaño.
  • La amplificación del xenón disuelto a granel permite la detección sensible de los analíticos.
  • Este método ofrece una nueva vía prometedora para la detección biomolecular sensible utilizando RMN.