Video Experimental Relacionado

Updated: Feb 20, 2026

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Visualizing Cellular Gibberellin Levels Using the nlsGPS1 Förster Resonance Energy Transfer (FRET) Biosensor

Published on: January 12, 2019

DWARF14 es un receptor hormonal no canónico para la estrigolactona

Ruifeng Yao, Zhenhua Ming, Liming Yan

|August 2, 2016

Resumen

La detección de la hormona vegetal estrigolactona incluye la proteína DWARF14 (D14). La hidrólisis de la estrigolactona genera una molécula intermedia vinculada covalentemente (CLIM), que activa el D14

Área de la Ciencia:

Biología vegetal
Señales moleculares
Receptores de las hormonas

Sus antecedentes:

Los receptores hormonales clásicos se unen a las hormonas no covalentemente para iniciar la transducción de señales.
DWARF14 (D14) es una hidrolasa alfa/beta involucrada en la hidrólisis de la estrigolactona y en la interacción con D3/MAX2.
El mecanismo preciso de la percepción de la estrigolactona y la identidad de la forma activa de la hormona siguen siendo elusivo.

Objetivo del estudio:

Esclarecer la base estructural de la percepción de la estrigolactona mediante la identificación de la forma activa de la hormona y el receptor genuino.
Investigar el papel de la hidrólisis de la estrigolactona en la activación de las vías de señalización.
Para comprender la doble función de D14 en la señalización de la estrigolactona.

Principales métodos:

Determinación de la estructura cristalina del complejo Arabidopsis thaliana D14-D3-ASK1 inducido por la estrigolactona.
Ensayos bioquímicos para analizar la hidrólisis de la estrigolactona y las interacciones con las proteínas.
Análisis genético de mutantes de Arabidopsis con función D14 alterada.

Principales resultados:

La estructura cristalina revela que la Arabidopsis thaliana D14 (AtD14) sufre una transición de estado abierto a cerrado al unirse a la estrigolactona.
La estrigolactona se hidroliza en una molécula intermedia vinculada covalentemente (CLIM), lo que induce un cambio conformacional en AtD14, facilitando la interacción D3.
El mutante AtD14 (G158E) conserva la actividad hidrolítica pero no interactúa con D3/MAX2, lo que indica una pérdida de la función del receptor.

Conclusiones:

AtD14 se activa alostéricamente mediante la hidrólisis de la estrigolactona en CLIM, iniciando un cambio conformacional.
AtD14 actúa como un receptor hormonal no canónico con doble función: generar y detectar la forma activa de la estrigolactona.
Este estudio define un nuevo mecanismo para la activación de los receptores hormonales y la señalización de la estrigolactona en las plantas.

Más Videos Relacionados

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy

Detection of Ligand-activated G Protein-coupled Receptor Internalization by Confocal Microscopy

Published on: April 9, 2017

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Published on: November 15, 2013

Videos de Conceptos Relacionados

Intracellular Hormone Receptors

Intracellular Hormone Receptors

Lipid-soluble hormones diffuse across the plasma and nuclear membrane of target cells to bind to their specific intracellular receptors. These receptors act as transcription factors that regulate gene expression and protein synthesis in the target cell

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Plant Hormones

Plant Hormones

Plant hormones—or phytohormones—are chemical molecules that modulate one or more physiological processes of a plant. In animals, hormones are often produced in specific glands and circulated via the circulatory system. However, plants lack hormone-producing glands.

Regulation of Hormone Secretion

Regulation of Hormone Secretion

Regulation of hormone secretion is a finely tuned orchestration driven by various types of stimuli, encompassing neural, humoral, and hormonal signals. Environmental cues instigate neural stimuli, where action potentials traverse nerve fibers to reach their designated targets. An illustrative scenario is the body's response to stress, wherein the sympathetic nervous system releases epinephrine from the adrenal glands, inducing the well-known 'fight or flight' reaction.
Humoral...

Secondary Messengers in Hormone Action

Secondary Messengers in Hormone Action

Water-soluble hormones cannot cross the plasma membrane, so they rely on protein receptors that span the membrane to trigger intracellular signaling pathways. These pathways then activate second messengers inside the cell, including cAMP or calcium ions.
Many hormones bind to transmembrane G protein-coupled receptors that connect to regulatory G proteins. These G proteins can then activate enzymes such as adenylyl cyclase or phospholipase C. Adenylyl cyclase converts ATP to cAMP, activating...

Endocrine Signaling

Endocrine Signaling

Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.