Video Experimental Relacionado

Updated: Mar 15, 2026

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

Published on: August 1, 2016

Consecuencias involuntarias de la expansión del alfabeto genético

Marvin Pollum¹, Brennan Ashwood¹, Steffen Jockusch²

¹Department of Chemistry, Case Western Reserve University , Cleveland, Ohio 44106, United States.

Journal of the American Chemical Society

|August 27, 2016

Resumen

El nuevo par de bases de ADN, d5SICS·dNaM, puede causar daño celular cuando se expone a la luz. Esto pone de relieve los riesgos potenciales de fototoxicidad asociados con la expansión del alfabeto genético para nuevas funciones biológicas.

Área de la Ciencia:

Biología sintética
La fotoquímica
Biología molecular

Sus antecedentes:

Una cepa modificada de Escherichia coli fue diseñada para incorporar y replicar el par de bases d5SICS·dNaM, creando el primer organismo semisintético estable.
Este alfabeto genético expandido tiene potencial para aumentar el almacenamiento de información y la traducción de proteínas novedosas.

Objetivo del estudio:

Investigar las propiedades fotoquímicas de los nucleósidos d5SICS y dNaM.
Evaluar los efectos fototóxicos potenciales de estos nuevos componentes del ADN.

Principales métodos:

Excitación de d5SICS y dNaM con luz casi visible.
Análisis de las poblaciones de estado triplet excitado.
Evaluación de la fotosensibilización celular, generación de especies reactivas de oxígeno y proliferación celular.

Principales resultados:

La excitación de d5SICS o dNaM pobla eficientemente sus estados triplet excitados.
Estos estados triplet de larga vida fotosensibilizan las células tras la fotoactivación.
La fotosensibilización conduce a la generación de especies reactivas de oxígeno y reduce significativamente la proliferación celular.

Conclusiones:

El par de bases d5SICS·dNaM exhibe propiedades fototóxicas debido a la formación de estados triplet excitados de larga duración.
Los científicos deben considerar estos efectos secundarios fototóxicos cuando utilizan alfabetos genéticos ampliados.

Más Videos Relacionados

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli

Published on: December 9, 2017

Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine DOPA and Its Application to Protein Conjugation

Genetic Incorporation of Biosynthesized L-dihydroxyphenylalanine DOPA and Its Application to Protein Conjugation

Published on: August 24, 2018

Videos de Conceptos Relacionados

From DNA to Protein

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...

Genome Copying Errors

Genome Copying Errors

DNA replication is a well-evolved process that copies millions of base pairs with high fidelity during each cell division. Occasionally a wrong base or a long stretch of wrong bases may get added to the daughter strands. If the errors are left unchecked, cells might accumulate several mutations that might endanger their survival. Therefore, the copying errors are checked and repaired at three levels.

The Central Dogma

The Central Dogma

The Central Dogma

The Central Dogma

The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...

Mutations

Mutations

Mutations

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...