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Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Se requieren dos genes estructurales en diferentes cromosomas para codificar la subunidad principal de la
1Department of Biochemical Genetics, Beckman Research Institute of the City of Hope, Duarte, California 91010.
Cell
|August 11, 1989
Resumen
La síntesis de glucosa-6-fosfato deshidrogenasa (G6PD) en glóbulos rojos humanos involucra dos subunidades con regiones de COOH compartidas pero con términos de NH2 distintos. Un segundo gen en el cromosoma 6 contribuye a la subunidad principal, lo que indica una regulación genética compleja.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Genética Humana Genética Humana.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La glucosa-6-fosfato deshidrogenasa (G6PD) de las células rojas humanas es crucial para el metabolismo celular.
- Estudios previos sugirieron un solo gen ligado a X para G6PD.
Objetivo del estudio:
- Para investigar la base estructural de la heterogeneidad de los glóbulos rojos humanos G6PD.
- Para identificar los orígenes genéticos de las diferentes subunidades G6PD.
Principales métodos:
- Análisis estructural de las subunidades G6PD.
- clonación de ADNc y caracterización de genes.
- Hibridación de manchas del sur y del norte.
Principales resultados:
- Se identificaron dos tipos de subunidades G6PD, que difieren en las regiones NH2-terminal.
- El NH2-terminal de la subunidad principal está codificado por un gen en el cromosoma 6, no el cromosoma X.
- Se detectaron dos componentes distintos de ARNm, uno para la región terminal de COOH y otro para la región terminal de NH2.
Conclusiones:
- La síntesis de glóbulos rojos humanos G6PD es un proceso complejo que involucra al menos dos genes separados.
- El gen G6PD vinculado a X y un gen vinculado al cromosoma 6 co-regulan la formación de subunidades G6PD.
- Es probable que mecanismos como la trans-traducción o la traducción cruzada medien la síntesis de la subunidad completa G6PD.
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