Search research articles

ACERCA DE JoVE

Visión General Liderazgo Blog Centro de Ayuda JoVE

AUTORES

Proceso de Publicación Consejo Editorial Alcance y Políticas Revisión por Pares Preguntas Frecuentes Enviar

BIBLIOTECARIOS

Testimonios Suscripciones Acceso Recursos Consejo Asesor de Bibliotecas Preguntas Frecuentes

INVESTIGACIÓN

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archivo

EDUCACIÓN

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Centro de Recursos para Profesores Sitio de Profesores

Términos y Condiciones de Uso

Política de Privacidad

Videos de Conceptos Relacionados

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

The Tumor Microenvironment

The Tumor Microenvironment

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

Cell-mediated Immune Responses

Cell-mediated Immune Responses

Immune Surveillance by NK Cells and Phagocytes

Immune Surveillance by NK Cells and Phagocytes

Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por

Same author

A phase 1 trial of HPV16 E7 T-cell receptor-engineered T cells in patients with relapsed/refractory HPV16-positive cancers (KITE-439 trial).

Frontiers in oncology·2026

Same author

HLA micropolymorphisms confine neoantigen conformational adaptability and guide T cell receptor selectivity.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Author Correction: The efficacy of chemotherapy is limited by intratumoral senescent cells expressing PD-L2.

Nature cancer·2026

Same author

Cancer type-specific variation in patterns of driver alterations across 50,000 tumors.

Cancer cell·2026

Same author

Mitochondrial ATP production promotes T cell differentiation and function by regulating chromatin accessibility.

bioRxiv : the preprint server for biology·2026

Same author

What factors differentiate early from late pulmonary metastases in soft-tissue sarcoma of the limbs? : a comparative cohort analysis.

The bone & joint journal·2026

Same journal

Keep the Hubble and James Webb Space Telescopes alive - the science is worth the price tag.

Nature·2026

Same journal

Say hello to hard helium.

Nature·2026

Same journal

How to avoid dementia - what the science really says.

Nature·2026

Same journal

Save Hubble: the race to preserve the space telescope kicks off.

Nature·2026

Same journal

How long can humans live? All evidence points to a maximum of 125 years.

Nature·2026

Same journal

Listen to Gen Z when it comes to AI in education.

Nature·2026

Ver todos los artículos relacionados

Search research articles

Video Experimental Relacionado

Updated: Mar 15, 2026

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

La supresión inmune iónica dentro del microambiente tumoral limita la función del efector de las células T

Robert Eil¹, Suman K Vodnala¹, David Clever¹

¹National Cancer Institute, National Institutes of Health (NIH), Bethesda, Maryland 20892, USA.

|September 15, 2016

Resumen

Este resumen es generado por máquina.

La necrosis tumoral libera potasio, suprimiendo la función de las células T. La sobreexpresión del canal K_v1.3 en las células T aumenta la inmunidad antitumoral y mejora la supervivencia en modelos de melanoma.

Más Videos Relacionados

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Published on: March 7, 2025

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Published on: April 13, 2015

Videos de Experimentos Relacionados

Last Updated: Mar 15, 2026

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Analysis of Human T Cell Activity in an Allogeneic Co-Culture Setting of Pre-Treated Tumor Cells

Published on: March 7, 2025

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model

Published on: April 13, 2015

Área de la Ciencia:

Inmunología
Biología del cáncer
Fisiología celular

Sus antecedentes:

La progresión tumoral ocurre a pesar de la infiltración de las células T.
La necrosis celular en los tumores se correlaciona con una baja supervivencia del paciente.
La necrosis libera componentes intracelulares que pueden afectar el microambiente del tumor.

Objetivo del estudio:

Investigar el impacto del potasio extracelular inducido por la necrosis en la función de las células T.
Para aclarar los mecanismos moleculares subyacentes a la supresión de las células T mediada por el potasio.
Explorar estrategias terapéuticas dirigidas a los niveles de potasio para la inmunoterapia del cáncer.

Principales métodos:

Análisis de la concentración de potasio extracelular (K+) en tumores de ratón y humanos.
Evaluación de la fosforilación de Akt-mTOR impulsada por el receptor de células T (TCR).
Ensayos funcionales de los programas efectores de células T in vitro e in vivo.
Manipulación genética de las células T para sobreexpresar el canal de potasio K_v1.3.

Principales resultados:

El potasio extracelular elevado ([K+]e) de la necrosis suprime la función del efector de las células T.
El alto [K+]e afecta la señalización de Akt-mTOR a través de la fosfatasa PP2A.
Se requiere un aumento del potasio intracelular ([K+]i) para la supresión, independiente del potencial de la membrana plasmática (Vm).
La sobreexpresión de K_v1.3 mejora la función de las células T y el aclaramiento del tumor en modelos de melanoma.

Conclusiones:

La hipercalemia inducida por necrosis crea un "punto de control iónico" que inhibe las respuestas de las células T antitumorales.
Dirigirse al eflujo de potasio representa una nueva estrategia para mejorar la inmunoterapia contra el cáncer.
La modulación de los niveles de potasio en las células T puede mejorar la erradicación del tumor y la supervivencia del paciente.