Video Experimental Relacionado

Updated: Mar 15, 2026

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis

The Citrobacter rodentium Mouse Model: Studying Pathogen and Host Contributions to Infectious Colitis

Published on: February 19, 2013

Los factores de virulencia aumentan la expansión de Citrobacter rodentium a través de la respiración aeróbica

Christopher A Lopez¹, Brittany M Miller¹, Fabian Rivera-Chávez¹

¹Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, One Shields Avenue, Davis, CA, USA.

Science (New York, N.Y.)

|September 17, 2016

Resumen

La infección por Citrobacter rodentium causa hiperplasia de la cripta del colon, aumentando el oxígeno y promoviendo el crecimiento de patógenos. La inhibición de esta hiperplasia reduce la ventaja competitiva del patógeno al limitar la respiración aeróbica.

Área de la Ciencia:

Microbiología
Gastroenterología
Interacciones del patógeno con el huésped

Sus antecedentes:

Citrobacter rodentium utiliza un sistema de secreción de tipo III (T3SS) para inducir la hiperplasia de la cripta del colon en ratones.
El mecanismo por el cual esta hiperplasia proporciona una ventaja competitiva frente a la microbiota intestinal sigue sin estar claro.

Objetivo del estudio:

Esclarecer el mecanismo por el cual la hiperplasia de la cripta del colon inducida por C. rodentium T3SS confiere una ventaja de aptitud.
Investigar el papel de la respiración aeróbica en la patogénesis de C. rodentium.

Principales métodos:

Inducción de la hiperplasia de la cripta del colon en ratones utilizando C. rodentium T3SS.
Evaluación de la proliferación de células epiteliales (células Ki67-positivas) y oxigenación de la mucosa.
Tratamiento con un inhibidor de la γ-secretasa (dibenzazepina) para bloquear la hiperplasia inducida por Notch.
Evaluación de la respiración aeróbica y la aptitud del C. rodentium.

Principales resultados:

La hiperplasia inducida por C. rodentium T3SS condujo a una expansión excesiva de las células epiteliales no diferenciadas.
Esta expansión aumentó la oxigenación de la mucosa, favoreciendo el crecimiento aeróbico de C. rodentium.
La inhibición de la hiperplasia con dibenzazepina redujo la ventaja de aptitud del patógeno relacionada con la respiración aeróbica.

Conclusiones:

C. rodentium emplea su T3SS para crear lesiones histopatológicas en el colon.
Estas lesiones generan un microambiente intestinal que apoya el crecimiento de patógenos a través de la respiración aeróbica.
La hiperplasia dirigida podría ser una estrategia para interrumpir la patogénesis de C. rodentium.

Más Videos Relacionados

4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

Published on: August 13, 2013

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria

Published on: February 23, 2014

Videos de Conceptos Relacionados

Gene Regulation in Microbial Communities: Quorum Sensing

Gene Regulation in Microbial Communities: Quorum Sensing

Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...

Oxygen Requirements and Growth Patterns

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...

Bacterial Phylum Bacteroidota

Bacterial Phylum Bacteroidota

The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...

Intracellular Movement of Viruses and Bacteria

Intracellular Movement of Viruses and Bacteria

Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...

Microbial Nutrition

Microbial Nutrition

Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...