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piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

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PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
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The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual,  the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
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Piaget's Stage 1 of Cognitive Development01:14

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The sensorimotor stage, the initial phase of Jean Piaget's theory of cognitive development, spans the first two years of a child's life. During this period, infants actively engage with their surroundings, building cognitive awareness through direct interaction with the world. This interaction is primarily based on sensory perception and motor actions, allowing infants to gradually understand basic physical properties and predict how objects interact within their environment.
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Pinocytosis

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Cells use energy-requiring bulk transport mechanisms to transfer large particles, or large amounts of small particles, into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
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Pinocytosis00:38

Pinocytosis

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Cells use energy-requiring bulk transport mechanisms to transfer large particles or large numbers of small particles into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
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Binary Fission01:20

Binary Fission

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Fission is the division of a single entity into two or more parts, which regenerate into separate entities that resemble the original. Organisms in the Archaea and Bacteria domains reproduce using binary fission, in which a parent cell splits into two parts that can each grow to the size of the original parent cell. This asexual method of reproduction produces cells that are all genetically identical.
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Video Experimental Relacionado

Updated: Mar 14, 2026

A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals
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A Noninvasive Hair Sampling Technique to Obtain High Quality DNA from Elusive Small Mammals

Published on: March 13, 2011

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PIWI da un paso de gigante

Yibei Xiao1, Ailong Ke1

  • 1Department of Molecular Biology and Genetics, Cornell University, 251 Biotechnology Building, Ithaca, NY 14853, USA.

Cell
|October 8, 2016
PubMed
Resumen
Este resumen es generado por máquina.

Los pequeños ARN llamados piARN guían a las proteínas PIWI para silenciar los transposones en la reproducción animal. Los investigadores revelan la primera estructura cristalina de una proteína PIWI unida a un piRNA, ofreciendo nuevas ideas sobre este mecanismo de silenciamiento.

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Área de la Ciencia:

  • Biología molecular
  • La genética
  • Biología estructural

Sus antecedentes:

  • Los ARN que interactúan con PIWI (piARN) son cruciales para silenciar los elementos transponibles en las líneas germinales animales.
  • El complejo de silenciamiento inducido por piRNA (piRISC) media este silenciamiento a través de una vía de biogénesis compleja.
  • La comprensión de la base estructural de la función de la proteína PIWI es clave para aclarar el montaje y la actividad de piRISC.

Objetivo del estudio:

  • Determinar la primera estructura cristalina de una proteína del clado PIWI unida a un ARN guía.
  • Proporcionar información estructural sobre el estado de preacción de las proteínas PIWI en el complejo piRISC.

Principales métodos:

  • Se empleó la cristalografía de rayos X para obtener la estructura de alta resolución.
  • Se utilizaron técnicas bioquímicas y biofísicas para caracterizar el complejo de ARN guía PIWI.

Principales resultados:

  • La estructura cristalina revela una proteína PIWI en una conformación preparada para la actividad silenciosa.
  • La estructura aclara la interacción precisa entre la proteína PIWI y el piRNA guía.

Conclusiones:

  • Este estudio estructural proporciona una comprensión fundamental de las interacciones del ARN guía PIWI.
  • Los hallazgos allanan el camino para futuras investigaciones sobre los mecanismos de formación y función de los piRISC.