Video Experimental Relacionado
Updated: Mar 13, 2026
09:53
In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
Published on: June 15, 2018
8.0K
La transcripción del ARN no codificante controla la expresión de Hand2 y el desarrollo del corazón
Kelly M Anderson1,2, Douglas M Anderson1,2, John R McAnally1,2
1Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA.
Nature
|November 4, 2016
Resumen
Un nuevo ARN largo no codificante, upperhand (Uph), es esencial para mantener la expresión del gen Hand2, un factor crítico en el desarrollo del corazón. La interrupción de la transcripción de Uph conduce a defectos cardíacos graves y letalidad embrionaria en ratones.
Área de la Ciencia:
- Biología del desarrollo
- La genética
- Biología molecular
Sus antecedentes:
- HAND2 es un factor de transcripción clave que regula el desarrollo del corazón.
- Su expresión está controlada por potenciadores marcados por modificaciones de H3K27ac.
- La desregulación de HAND2 causa defectos cardíacos y letalidad embrionaria.
Objetivo del estudio:
- Investigar el papel de un nuevo ARN largo no codificante, upperhand (Uph), en la regulación de la expresión de Hand2.
- Aclarar el mecanismo por el cual el Uph influye en la actividad del potenciador Hand2.
- Comprender las consecuencias funcionales de la interrupción de la Uph en el desarrollo del corazón.
Principales métodos:
- Análisis de la transcripción de Uph y su impacto en la expresión de Hand2 en modelos de ratón.
- Investigación del papel de Uph en el mantenimiento de firmas de super-potenciadores.
- Evaluación de la elongación de la ARN polimerasa II a través del locus del potenciador Hand2.
- Análisis fenotípico de ratones con bloqueo de transcripción de Uph.
Principales resultados:
- Se requiere transcripción Uph para mantener la firma del super potenciador Hand2.
- Bloqueo de la transcripción de Uph, pero no de la transcripción madura, eliminación de la expresión de Hand2.
- La interrupción de Uph condujo a la hipoplasia del ventrículo derecho y la letalidad embrionaria en ratones.
- Uph facilita el alargamiento de la ARN polimerasa II a través del locus potenciador de Hand2.
Conclusiones:
- La asociación reguladora Uph-Hand2 destaca un nuevo mecanismo para controlar la expresión génica a través de ARN no codificantes.
- La transcripción no codificante divergente puede establecer entornos de cromatina permisivos cruciales para el desarrollo.
- Este hallazgo tiene implicaciones para la comprensión del desarrollo cardíaco y el papel de los lncRNA en la regulación genética.
Videos de Conceptos Relacionados
02:23Master Transcription Regulators
8.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
01:13Formation of Muscle Fibers from Myoblasts
6.3K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
6.3K
01:30General Transcription Factors
7.4K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.4K
01:22MicroRNAs
4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
01:22MicroRNAs
24.5K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.5K
01:17Transcription
34.7K
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
34.7K