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Fotoactivación e inactivación de Arabidopsis criptocromo 2

Qin Wang^1,2, Zecheng Zuo^1,3, Xu Wang^1,2

¹Basic Forestry and Proteomics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Science (New York, N.Y.)

|November 16, 2016

Resumen

La luz azul activa los criptocromos de las plantas (CRY2) a través de la dimerización. BIC1 inhibe este proceso, regulando la actividad de CRY2 y potencialmente controlando la homeostasis criptocromática entre las especies.

Área de la Ciencia:

Biología molecular de las plantas
Fotobiología
Los ritmos circadianos

Sus antecedentes:

Los criptocromos son fotorreceptores esenciales de luz azul que regulan el desarrollo de las plantas y los ritmos circadianos.
El criptocromo 2 (CRY2) en Arabidopsis requiere homodimerización dependiente de la luz azul para la actividad fisiológica.

Objetivo del estudio:

Para identificar los reguladores de la actividad del criptocromo.
Para investigar el mecanismo de la regulación CRY2 por la luz azul.

Principales métodos:

Cribado de dos híbridos de levadura para identificar las proteínas que interactúan con CRY2.
Pruebas de unión in vitro.
Análisis de la dimerización, fosforilación y degradación de CRY2 en el planta.

Principales resultados:

BIC1 (inhibidor de la luz azul de los criptocromos 1) fue identificado como un nuevo inhibidor de los criptocromos vegetales.
BIC1 se une directamente a CRY2 y suprime la homodimerización de CRY2 dependiente de la luz azul.
BIC1 inhibe la formación, fosforilación y degradación de los fotcuerpos mediados por CRY2, suprimiendo así las actividades fisiológicas de CRY2.

Conclusiones:

La dimerización regulada es un mecanismo clave que controla la actividad del criptocromo y la homeostasis en las plantas.
BIC1 actúa como un regulador negativo de CRY2, proporcionando un nuevo objetivo para comprender las vías de señalización de la luz.
La hipótesis de que la dimerización regulada rige la homeostasis criptocromática puede extenderse a otros linajes evolutivos.

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