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Tecnologías basadas en CRISPR para la manipulación de genomas de eucariotas

Alexis C Komor¹, Ahmed H Badran¹, David R Liu¹

¹Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02141, USA.

|November 22, 2016

Resumen

La tecnología de edición de genes CRISPR-Cas9 permite una modificación precisa del ADN en las células vivas. Esta revisión cubre las herramientas CRISPR para la edición del genoma de mamíferos y sus aplicaciones en investigación y medicina.

Área de la Ciencia:

Biología molecular
La genética
Biotecnología

Sus antecedentes:

CRISPR-Cas9 es una endonucleasa de ADN guiada por ARN.
Ha impulsado avances significativos en las ciencias de la vida al permitir la edición del genoma en células vivas.

Objetivo del estudio:

Revisar las tecnologías basadas en CRISPR para la edición del genoma de mamíferos.
Para resaltar sus diversas aplicaciones y desarrollos recientes.

Principales métodos:

Resumiendo las tecnologías basadas en CRISPR.
Describiendo los avances recientes en los sistemas CRISPR.
Destacando las aplicaciones en investigación básica, biotecnología y terapéutica.

Principales resultados:

Las tecnologías CRISPR se han mejorado para la generalidad, la especificidad del ADN y la selectividad del producto.
Estos avances han facilitado progresos notables en varios campos científicos.

Conclusiones:

Las tecnologías basadas en CRISPR son herramientas poderosas para la edición del genoma de mamíferos.
Tienen amplias aplicaciones y siguen impulsando la innovación en las ciencias de la vida y la medicina.

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