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NAD+ como un donante de hidrógeno y reductor

Yamin Htet, Andrew G Tennyson¹

¹Center for Optical Materials Science and Engineering Technologies , Anderson, South Carolina 29625, United States.

Journal of the American Chemical Society

|December 15, 2016

Resumen

El nicotinamida adenina dinucleótido oxidado (NAD +) ahora puede actuar como reductor, generando un intermediario de hidruro de rutenio. Este descubrimiento permite una mayor producción de peróxido de hidrógeno para posibles terapias contra el cáncer.

Área de la Ciencia:

Catálisis
Química bioorgánica
La bioquímica

Sus antecedentes:

El nicotinamida adenina dinucleótido reducido (NADH) genera un intermediario de hidruro de rutenio para la reducción de O2 a H2O2, que ofrece propiedades anticancerígenas.
El uso de nicotinamida adenina dinucleótido oxidada (NAD+) como fuente de H podría aumentar significativamente la producción de H2O2 debido a su mayor abundancia.

Objetivo del estudio:

Para demostrar que el NAD+ puede funcionar como reductor y donante de H-.
Investigar el mecanismo de la formación intermedia de Ru-H utilizando el NAD+.
Explorar aplicaciones potenciales en la terapia del cáncer y las vías biológicas.

Principales métodos:

Se utilizó la reducción catalítica de ABTS•- a ABTS2- para probar el NAD+ como reductor.
Se realizaron estudios mecanicistas para dilucidar la formación del intermediario hidruro de rutenio.

Principales resultados:

Se demostró que el NAD+ funcionaba eficazmente como reductor.
La evidencia sugiere la formación de un intermediario Ru-H a través de la eliminación de β-hidruro de NAD+.
Esta vía permite una producción de H2O2 significativamente mayor en comparación con el uso de NADH.

Conclusiones:

El NAD+ puede servir como reductor y donante de H, expandiendo el alcance de la química redox biológica conocida.
Los hallazgos pueden revelar nuevas vías biológicas de oxidación de carbohidratos.
Esta investigación abre caminos para el desarrollo de nuevas estrategias quimioterapéuticas.

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