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Un regulador global bacteriano forma un prión

Andy H Yuan1, Ann Hochschild2

  • 1Department of Microbiology and Immunobiology, Harvard Medical School, 4 Blackfan Circle, Boston, MA 02115, USA.

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|January 14, 2017
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Resumen
Este resumen es generado por máquina.

Las bacterias pueden formar priones, que son agregados de proteínas que se propagan por sí mismos. Los investigadores descubrieron una proteína bacteriana, Rho, que puede formar un prión, lo que sugiere que los priones existían antes de que los eucariotas y las bacterias divergieran.

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Área de la Ciencia:

  • Microbiología
  • Biología molecular
  • La bioquímica de las proteínas

Sus antecedentes:

  • Los priones son elementos de herencia conocidos basados en proteínas eucariotas, caracterizados por agregados de autopropagación.
  • Los priones no se han identificado previamente en sistemas bacterianos.
  • Comprender los orígenes y la distribución de los priones es crucial para la biología evolutiva.

Objetivo del estudio:

  • Para investigar el potencial de la formación de priones en las proteínas bacterianas.
  • Identificar y caracterizar las capacidades de formación de priones del terminador de transcripción Rho bacteriano (Cb-Rho).
  • Para explorar las implicaciones evolutivas del descubrimiento de priones bacterianos.

Principales métodos:

  • Identificación de un dominio formador de priones candidato (cPrD) dentro de Cb-Rho.
  • Ensayos funcionales en levaduras para comprobar la capacidad del cPrD para sustituir un dominio priónico conocido (PrD).
  • Expresión y caracterización de las conformaciones Cb-Rho en Escherichia coli, incluidas las formas de priones solubles y agregados.
  • Análisis transcriptómico para evaluar el impacto de la forma de prión Cb-Rho en la expresión génica de todo el genoma.

Principales resultados:

  • La proteína bacteriana Cb-Rho fue identificada como capaz de formar un prión.
  • Un cPrD específico en Cb-Rho confería amiloidogenicidad y podría sustituir funcionalmente a los PrD de levadura.
  • Cb-Rho adoptó conformaciones alternativas en E. coli: una forma soluble, transcripcionalmente activa y una forma priónica auto-propagadora, funcionalmente comprometida.
  • La forma priónica de Cb-Rho indujo cambios transcriptómicos significativos en todo el genoma.

Conclusiones:

  • Las proteínas bacterianas, como Cb-Rho, pueden funcionar como elementos de herencia basados en proteínas.
  • El descubrimiento de priones bacterianos sugiere que su aparición es anterior a la divergencia evolutiva de eucariotas y bacterias.
  • Este hallazgo amplía el alcance conocido de la biología priónica y su historia evolutiva.