JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us
Esta página ha sido traducida por una máquina. Otras páginas pueden seguir apareciendo en inglés. View in English

Modificadores de hábitos de cristal de ADN diseñados

  • 0Department of Chemistry & Biochemistry and Center for Biomolecular Structure and Organization, University of Maryland , College Park, Maryland 20742, United States.
Journal of the American Chemical Society +

|

18 de enero de 2017

|

18 de enero de 2017

Ver abstracta en PubMed

Resumen

Este resumen es generado por máquina.

Los investigadores ahora pueden controlar la forma de los cristales de ADN 3D utilizando oligonucleótidos "venenosos". Este avance en la nanotecnología del ADN permite hábitos de cristal a medida para aplicaciones avanzadas.

Área De La Ciencia

  • Nanotecnología del ADN
  • Ciencias de los materiales
  • Autoensamblaje a nanoescala

Sus Antecedentes

  • El ADN es una molécula versátil para crear estructuras a nanoescala.
  • Un objetivo clave es ensamblar cristales de ADN 3D macroscópicos para el posicionamiento preciso de moléculas.
  • Adaptar las propiedades del cristal, como la morfología, es crucial para la integración en sistemas complejos.

Objetivo Del Estudio

  • Para demostrar el control sobre los hábitos de los cristales de ADN 3D.
  • Investigar el uso de secuencias de ADN modificadas para alterar la morfología del cristal.
  • Para permitir la integración de cristales de ADN en sistemas más complejos.

Principales Métodos

  • Auto-ensamblaje de un cristal de ADN 3D utilizando un ADN 13-mer.
  • Introducción de oligonucleótidos "venenosos" para interrumpir las interacciones específicas de emparejamiento de bases.
  • Aplicación de oligonucleótidos venenosos durante la cristalización inicial y el crecimiento de la capa de la cáscara.

Principales Resultados

  • Se logró una modificación predecible de los hábitos de los cristales de ADN 3D.
  • Los oligonucleótidos venenosos alteraron la morfología de los cristales.
  • La modificación del hábito tuvo éxito tanto durante la formación inicial de cristales como en las fases de crecimiento posteriores.

Conclusiones

  • Los hábitos cristalinos de los cristales de ADN 3D se pueden alterar de manera predecible utilizando oligonucleótidos venenosos.
  • Este método permite morfologías de cristales de ADN a medida para diversas aplicaciones.
  • La técnica es aplicable tanto durante las etapas de cristalización primaria como de crecimiento secundario.

Videos de Conceptos Relacionados

Determination of Crystal Structures 01:29

24

In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...

Crystal Growth: Principles of Crystallization 01:25

5.5K

Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent...

Imperfections in Crystal Structure: Stoichiometric Point Defects 01:26

30

Schottky defects arise when some lattice points in a crystal, such as those in NaCl, remain unoccupied, creating lattice vacancies without disturbing the overall electrical neutrality of the crystal. This defect is common in ionic crystals where the positive and negative ions are similar in size, as seen in sodium chloride and cesium chloride. The presence of Schottky defects enables the crystal to conduct electricity to a small extent through an ionic mechanism. Electric fields cause nearby...

Imperfections in Crystal Structure: Point, Line and Plane Defects 01:25

24

A perfect crystal, in theory, has a uniform structure with the same unit cell and lattice points throughout. However, any deviation from this periodic arrangement is known as an imperfection or defect. These defects can be categorized into three types: point, line, and plane defects.Point defects occur when there is a deviation from the ideal due to missing atoms, displaced atoms, or additional atoms. These imperfections might occur due to imperfect packing during crystallization or because of...

X-ray Crystallography 02:18

26.6K

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

Imperfections in Crystal Structure: Non-Stoichiometric Defects 01:29

20

Non-stoichiometric defects refer to a type of defect in the crystal structure of a compound where the ratio of its constituent elements deviates from the ideal stoichiometric ratio. There are two main types of non-stoichiometric defects: metal excess defects and metal deficiency defects.Metal excess defects occur when there is a slight surplus of metal ions than what is required by the stoichiometric ratio of the compound. For example, heating a sodium chloride crystal in sodium vapor results...